caa a22 4500 445341238 CHVBK 20180317142813.0 cr unu---uuuuu 170323e20111101xx s 000 0 eng 10.1007/s00436-011-2429-x doi (NATIONALLICENCE)springer-10.1007/s00436-011-2429-x Cloning and characterization of the autophagy-related gene 6 from the hard tick, Haemaphysalis longicornis [Elektronische Daten] [Suguru Kawano, Rika Umemiya-Shirafuji, Damdinsuren Boldbaatar, Kenji Matsuoka, Tetsuya Tanaka, Kozo Fujisaki] Autophagy is the intracellular protein degradation process which is induced by starvation. Ticks have a unique tolerance for starvation, and it is possible that this tolerance is associated with their longevity. Previously, we isolated the homologues of four autophagy-related (ATG) genes in the hard tick, Haemaphysalis longicornis, suggesting that autophagy appeared to play an important role in tolerance for starvation as well as the development of ticks. In this study, the homologue of ATG6 was isolated from H. longicornis (HlATG6). HlATG6 mRNA expression was higher in the egg and unfed larval stages than in other stages and upregulated in ovaries during the blood-feeding period. Moreover, HlATG6-knockdowned ticks laid a few and poorly developed eggs that were white brown in color and not well surface-coated with wax. However, the expression of vitellogenin (Vg)-2, HlVg-2, in the fat body of HlATG6-knockdowned ticks was significantly upregulated. In addition, hemolymph had a deep brown color in HlATG6-knockdowned ticks on day21 after engorgement and drop-off, indicating that the Vgs synthesized by the fat body and midgut are retained and accumulated in the hemolymph of HlATG6-knockdowned ticks, probably due to the downregulation of the Vg uptake capability of oocytes. Interestingly, HlATG6 knockdown provided non-significant influences on the expression of the Vg receptor (HlVgR) at oocytes, suggesting a non-significant depression of VgR-mediated endocytosis in the oocytes of HlATG6-knockdowned ticks. Therefore, it was interpreted that the repression of Vg uptake in the oocytes of HlATG6-knockdowned ticks may be involved in endocytic processes other than the receptor recognition of Vgs in oocytes. Springer-Verlag, 2011 Kawano Suguru Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan aut Umemiya-Shirafuji Rika Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan aut Boldbaatar Damdinsuren Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan aut Matsuoka Kenji Matsuoka Research Institute, Midori-machi 5-19-21, Koganei, 184-0003, Tokyo, Japan aut Tanaka Tetsuya Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan aut Fujisaki Kozo Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan aut Parasitology Research Springer-Verlag 109/5(2011-11-01), 1341-1349 0932-0113 109:5<1341 2011 109 436 https://doi.org/10.1007/s00436-011-2429-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2429-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawano Suguru Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 700 1- Umemiya-Shirafuji Rika Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 700 1- Boldbaatar Damdinsuren Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 700 1- Matsuoka Kenji Matsuoka Research Institute, Midori-machi 5-19-21, Koganei, 184-0003, Tokyo, Japan aut NATIONALLICENCE 700 1- Tanaka Tetsuya Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 700 1- Fujisaki Kozo Laboratory of Emerging Infectious Diseases, Department of Frontier Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Korimoto 1-21-24, 890-0065, Kagoshima, Japan aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/5(2011-11-01), 1341-1349 0932-0113 109:5<1341 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer