caa a22 4500 44534136X CHVBK 20180317142814.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2277-8 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2277-8 Acaricidal, pediculocidal and larvicidal activity of synthesized ZnO nanoparticles using wet chemical route against blood feeding parasites [Elektronische Daten] [Arivarasan Kirthi, Abdul Rahuman, Govindasamy Rajakumar, Sampath Marimuthu, Thirunavukkarasu Santhoshkumar, Chidambaram Jayaseelan, Kanayairam Velayutham] The present study was based on assessments of the anti-parasitic activities to determine the efficacies of synthesized zinc oxide nanoparticles (ZnO NPs) prepared by wet chemical method using zinc nitrate and sodium hydroxide as precursors and soluble starch as stabilizing agent against the larvae of cattle tick Rhipicephalus (Boophilus) microplus, Canestrini (Acari: Ixodidae); head louse Pediculus humanus capitis, De Geer (Phthiraptera: Pediculidae); larvae of malaria vector, Anopheles subpictus, Grassi; and filariasis vector, Culex quinquefasciatus, Say (Diptera: Culicidae). R. microplus larvae were exposed to filter paper envelopes impregnated with different ZnO NP concentrations. Direct contact method was conducted to determine the potential of pediculocidal activity. Parasite larvae were exposed to varying concentrations of synthesized ZnO NPs for 24h. The results suggested that the mortality effects of synthesized ZnO NPs were 43% at 1h, 64% at 3h, 78% at 6h, and 100% after 12h against R. microplus activity. In pediculocidal activity, the results showed that the optimal times for measuring mortality effects of synthesized ZnO NPs were 38% at 10min, 71% at 30min, 83% at 1h, and 100% after 6h against P. humanus capitis. One hundred percent lice mortality was observed at 10mg/L treated for 6h. The mortality was confirmed after 24h of observation period. The larval mortality effects of synthesized ZnO NPs were 37%, 72%, 100% and 43%, 78% and 100% at 6, 12, and 24h against A. subpictus and C. quinquefasciatus, respectively. It is apparent that the small size and corresponding large specific surface area of small nanometer-scale ZnO particles impose several effects that govern its parasitic action, which are size dependent. ZnO NPs were synthesized by wet chemical process, and it was characterized with the UV showing peak at 361nm. X-ray diffraction (XRD) spectra clearly shows that the diffraction peaks in the pattern indexed as the zinc oxide with lattice constants a = 3.249 and c = 5.206Å. The FTIR spectrum showed the range of 400-4,000cm−1. The band at 899.56cm−1; 1,151.87cm−1; 1,396cm−1; and these bands showed the complete composition of ZnO NPs. SEM micrograph showed 60-120-nm size and aggregates of spherical shape nanoparticles. EDX showed the complete chemical composition of the synthesized nanoparticles of zinc oxide. The maximum efficacy was observed in zinc oxide against the R. microplus, P. humanus capitis, and the larvae of A. subpictus, C. quinquefasciatus with LC50 values of 29.14, 11.80, 11.14, and 12.39mg/L; r 2 = 0.805, 0.876, 0.894, and 0.904, respectively. The synthesized ZnO NPs showed the LC50 and r 2 values against the R. microplus (13.41mg/L; 0.982), P. humanus capitis (11.80mg/L; 0.966), and the larvae of A. subpictus (3.19; 0.945mg/L), against C. quinquefasciatus (4.87mg/L; 0.970), respectively. The control (distilled water) showed nil mortality in the concurrent assay. This is the first report on anti-parasitic activity of the synthesized ZnO NPs. Springer-Verlag, 2011 Kirthi Arivarasan Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut Rahuman Abdul Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut Rajakumar Govindasamy Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut Marimuthu Sampath Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut Santhoshkumar Thirunavukkarasu Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut Jayaseelan Chidambaram Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut Velayutham Kanayairam Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut Parasitology Research Springer-Verlag 109/2(2011-08-01), 461-472 0932-0113 109:2<461 2011 109 436 https://doi.org/10.1007/s00436-011-2277-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2277-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kirthi Arivarasan Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut NATIONALLICENCE 700 1- Rahuman Abdul Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut NATIONALLICENCE 700 1- Rajakumar Govindasamy Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut NATIONALLICENCE 700 1- Marimuthu Sampath Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut NATIONALLICENCE 700 1- Santhoshkumar Thirunavukkarasu Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut NATIONALLICENCE 700 1- Jayaseelan Chidambaram Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut NATIONALLICENCE 700 1- Velayutham Kanayairam Unit of Nanotechnology and Bioactive Natural Products, Post Graduate and Research Department of Zoology, C. Abdul Hakeem College, Melvisharam-632 509, Vellore District, Tamil Nadu, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/2(2011-08-01), 461-472 0932-0113 109:2<461 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer