caa a22 4500 445341416 CHVBK 20180317142814.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2253-3 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2253-3 Early detection and estimation of infection burden by real-time PCR in rats experimentally infected with Clonorchis sinensis [Elektronische Daten] [S. Rahman, Young Bae, Sung-Tae Hong, Min-Ho Choi] A real-time PCR assay targeting the cytochrome c oxidase gene of Clonorchis sinensis was developed and its diagnostic value was investigated in experimentally infected rats. The PCR assay amplified as little as 0.1pg of C. sinensis genomic DNA and a single egg from experimentally spiked negative feces. The assay could detect C. sinensis DNA in feces 2weeks post-infection (PI), whereas the Kato-Katz method and formalin-ether concentration techniques could detect the eggs in the feces from 3weeks PI. Inverse correlations between eggs per gram of feces (EPG) and Ct values were observed 4, 6, and 8weeks PI. Worm burden was positively correlated with EPG but negatively correlated with Ct values in different groups at 8weeks PI. Fecal real-time PCR provides a potential tool for the diagnosis of clonorchiosis before eggs can be detected by conventional methods, and may be used to estimate the worm burden in the host. Springer-Verlag, 2011 Rahman S. Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea aut Bae Young Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea aut Hong Sung-Tae Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea aut Choi Min-Ho Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea aut Parasitology Research Springer-Verlag 109/2(2011-08-01), 297-303 0932-0113 109:2<297 2011 109 436 https://doi.org/10.1007/s00436-011-2253-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2253-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rahman S. Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea aut NATIONALLICENCE 700 1- Bae Young Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea aut NATIONALLICENCE 700 1- Hong Sung-Tae Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea aut NATIONALLICENCE 700 1- Choi Min-Ho Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, 110-799, Seoul, South Korea aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/2(2011-08-01), 297-303 0932-0113 109:2<297 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer