caa a22 4500 445341424 CHVBK 20180317142814.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2273-z doi (NATIONALLICENCE)springer-10.1007/s00436-011-2273-z Trypanosoma cruzi strains in the Calomys callosus [Elektronische Daten] parasitemia and reaction of intracellular forms with stage-specific antibodies in the acute and chronic phase of infection and after immunosuppression [Noemi Taniwaki, Viviane Gonçalves, Julianna Romero, Claudio da Silva, Solange da Silva, Renato Mortara] An experimental model for chronic Chagas disease was developed to investigate whether reactivation is influenced by the genetic origin of Trypanosoma cruzi isolates. In addition, we examined whether the distribution of T. cruzi stage-specific epitopes, as defined by monoclonal antibodies (Mab), raised against mammalian-stage parasite forms, exhibited comparable distribution patterns in Calomys callosus myocardium during the acute phase and after reactivation of the infection. Animals were infected with parasites of the G (T. cruzi I), Y (T.cruzi II) or CL strains (T. cruzi VI). Heart sections were labelled with the Mabs 2C2, 1D9, 2B7, 3B9 and 4B9, which react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein. Mab 1D9 and 2B7 showed polymorphic distributions over amastigotes among animals infected with the G, Y or CL strains. Mab 3B2, which recognises a non-carbohydrate epitope in flagellated forms, showed an active state of parasite dissemination in the myocardium of C. callosus that were infected with Y or CL strains and then immunosuppressed after 6 or 12months. C. callosus infected with the G strain (T. cruzi I) displayed absence of amastigote nests in the heart after immunosuppression. Our results permit us to suggest that parasites of the G strain may be more sensitive to the immune response, since we could not find either evidence of parasitemia or amastigote nests. Conversely, parasites from the Y and CL strains appeared able to escape the immune response, as evidenced by an inflammatory infiltrate and disseminated infection after immunosuppression. Springer-Verlag, 2011 Taniwaki Noemi Electron Microscopy Unit, Adolfo Lutz Institute, Av. Dr. Arnaldo 355, 01246-902, São Paulo (SP), Brazil aut Gonçalves Viviane Electron Microscopy Unit, Adolfo Lutz Institute, Av. Dr. Arnaldo 355, 01246-902, São Paulo (SP), Brazil aut Romero Julianna Electron Microscopy Unit, Adolfo Lutz Institute, Av. Dr. Arnaldo 355, 01246-902, São Paulo (SP), Brazil aut da Silva Claudio Department of Immunology, Biomedical Sciences Institute, Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Minas Gerais, Brazil aut da Silva Solange Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo - UNIFESP, R. Botucatu, 862 6˚ andar, 04023-062, São Paulo, Brazil aut Mortara Renato Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo - UNIFESP, R. Botucatu, 862 6˚ andar, 04023-062, São Paulo, Brazil aut Parasitology Research Springer-Verlag 109/2(2011-08-01), 431-440 0932-0113 109:2<431 2011 109 436 https://doi.org/10.1007/s00436-011-2273-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2273-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taniwaki Noemi Electron Microscopy Unit, Adolfo Lutz Institute, Av. Dr. Arnaldo 355, 01246-902, São Paulo (SP), Brazil aut NATIONALLICENCE 700 1- Gonçalves Viviane Electron Microscopy Unit, Adolfo Lutz Institute, Av. Dr. Arnaldo 355, 01246-902, São Paulo (SP), Brazil aut NATIONALLICENCE 700 1- Romero Julianna Electron Microscopy Unit, Adolfo Lutz Institute, Av. Dr. Arnaldo 355, 01246-902, São Paulo (SP), Brazil aut NATIONALLICENCE 700 1- da Silva Claudio Department of Immunology, Biomedical Sciences Institute, Federal University of Uberlândia, Av. Pará, 1720, 38400-902, Minas Gerais, Brazil aut NATIONALLICENCE 700 1- da Silva Solange Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo - UNIFESP, R. Botucatu, 862 6˚ andar, 04023-062, São Paulo, Brazil aut NATIONALLICENCE 700 1- Mortara Renato Department of Microbiology, Immunology and Parasitology, Federal University of São Paulo - UNIFESP, R. Botucatu, 862 6˚ andar, 04023-062, São Paulo, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/2(2011-08-01), 431-440 0932-0113 109:2<431 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer