caa a22 4500 445341432 CHVBK 20180317142814.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2266-y doi (NATIONALLICENCE)springer-10.1007/s00436-011-2266-y Differential miRNA expression in the mouse jejunum during garlic treatment of Eimeria papillata infections [Elektronische Daten] [Saleh Al-Quraishy, Denis Delic, Helmult Sies, Frank Wunderlich, Abdel Abdel-Baki, Mohamed Dkhil] Accumulating evidence indicates a critical role of microRNAs (miRNAs) in the outcome of diseases. Here, we investigate the effect of garlic on the intestinal miRNA signature of male Balb/c mice during infections with Eimeria papillata. Garlic decreases the intracellular development as evidenced by a lowered fecal output of E. papillata oocysts from 3,150 ± 410 to approximately 1,750 ± 390 oocysts per gram feces on day4 postinoculation. This anti-coccidial activity of garlic is associated with an inhibition of the E. papillata-induced increases of interferon gamma, inducible nitric oxide synthase, nitrite/nitrate, and malondialdehyde and decrease in glutathione. Moreover, garlic downregulates the E. papillata-induced increases in the expression of the miRNAs miR-1959, miR-203, and miR-21, and it upregulates the expression of the 11 miRNA species miR-142-5P, miR-15A, miR-10A, miR-29B, miR-1902, miR-125A-5P, let-7E, miR-148A, miR-130A, miR-10B, and miR-93, respectively, as revealed by miRXplore microarray technology. Real-time PCR confirms these effects of garlic in the jejunum of E. papillata-infected mice. Our data indicate that the anti-coccidial activity of garlic is associated with specific changes in the miRNA signature of the mouse jejunum, the target site of E. papillata. These changes may reflect an involvement of miRNAs in garlic-activated pathways to reduce and/or to repair E. papillata-induced tissue injuries. Springer-Verlag, 2011 Al-Quraishy Saleh Department of Zoology, College of Science, King Saud University, P.O. Box2455, 11451, Riyadh, Saudi Arabia aut Delic Denis Department of Molecular Parasitology, Heinrich Heine University, 40225, Duesseldorf, Germany aut Sies Helmult Department of Zoology, College of Science, King Saud University, P.O. Box2455, 11451, Riyadh, Saudi Arabia aut Wunderlich Frank Department of Zoology, College of Science, King Saud University, P.O. Box2455, 11451, Riyadh, Saudi Arabia aut Abdel-Baki Abdel Department of Zoology, College of Science, King Saud University, P.O. Box2455, 11451, Riyadh, Saudi Arabia aut Dkhil Mohamed Department of Zoology, College of Science, King Saud University, P.O. Box2455, 11451, Riyadh, Saudi Arabia aut Parasitology Research Springer-Verlag 109/2(2011-08-01), 387-394 0932-0113 109:2<387 2011 109 436 https://doi.org/10.1007/s00436-011-2266-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2266-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Al-Quraishy Saleh Department of Zoology, College of Science, King Saud University, P.O. Box2455, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Delic Denis Department of Molecular Parasitology, Heinrich Heine University, 40225, Duesseldorf, Germany aut NATIONALLICENCE 700 1- Sies Helmult Department of Zoology, College of Science, King Saud University, P.O. Box2455, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Wunderlich Frank Department of Zoology, College of Science, King Saud University, P.O. Box2455, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Abdel-Baki Abdel Department of Zoology, College of Science, King Saud University, P.O. Box2455, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Dkhil Mohamed Department of Zoology, College of Science, King Saud University, P.O. Box2455, 11451, Riyadh, Saudi Arabia aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/2(2011-08-01), 387-394 0932-0113 109:2<387 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer