caa a22 4500 445341483 CHVBK 20180317142814.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2259-x doi (NATIONALLICENCE)springer-10.1007/s00436-011-2259-x Proteaese activity of Blastocystis hominis subtype3 in symptomatic and asymptomatic patients [Elektronische Daten] [Dina Abdel-Hameed, Omayma Hassanin] Despite accumulating evidence indicating that Blastocystis hominis is pathogenic and that cysteine proteases are involved in its pathogenesis, few researches discussed the protease activity of B. hominis genetic subtypes. Therefore, the present study aims to identify the underlying pathogenic role of the proteases of B. hominis subtype 3 at different molecular weights in correlation to gastrointestinal symptoms. Of 65 patients with various clinical presentations referred to our laboratory for stool examination, 26 (40%) were B. hominis positive by stool culture. Of 26 (group I) B. hominis patients, 18 (69.2%) were symptomatic (group IA) and 8(30.8%) were asymptomatic (group IB). Of 25 normal control group (group II), 5 (20%) were B. hominis positive. Subtype 3 was the only genotype recovered by polymerase chain reaction. Of 26 patients in group I, 19 (73.1%) were immunocompetent and 7 (26.9%) were immunocompromised. Protease activities of B. hominis subtype 3 were recognized at 32-kDa (46.2%), 39-kDa (7.7%), 120-kDa (38.5%), 140-kDa (11.5%), and 215-kDa (19.2%) bands in gelatin sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Proteases were recognized in 17 (94.4%) out of 18 symptomatic Blastocystis patients versus 2 (25.0%) out of 8 asymptomatic patients. Proteases at 32kDa were reported in 61.1% of symptomatic versus 12.5% of asymptomatic patients. It was concluded that proteases of B. hominis genetic subtype 3, particularly those at 32kDa, could be considered a virulence factor that is responsible for protein degradation and have a possible pathogenic role in host immune evasion. Springer-Verlag, 2011 Abdel-Hameed Dina Department of Parasitology, Faculty of Medicine, Ain Shams University, 13 B Kornesh El Neel, Aghkhan, Shobra, Cairo, Egypt aut Hassanin Omayma Clinical Pathology, Molecular Biology Department, Medical Research Center, Faculty of Medicine, Ain-Shams University Hospitals, Abbassia, P.O.11361, Cairo, Egypt aut Parasitology Research Springer-Verlag 109/2(2011-08-01), 321-327 0932-0113 109:2<321 2011 109 436 https://doi.org/10.1007/s00436-011-2259-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2259-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abdel-Hameed Dina Department of Parasitology, Faculty of Medicine, Ain Shams University, 13 B Kornesh El Neel, Aghkhan, Shobra, Cairo, Egypt aut NATIONALLICENCE 700 1- Hassanin Omayma Clinical Pathology, Molecular Biology Department, Medical Research Center, Faculty of Medicine, Ain-Shams University Hospitals, Abbassia, P.O.11361, Cairo, Egypt aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/2(2011-08-01), 321-327 0932-0113 109:2<321 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer