caa a22 4500 445341505 CHVBK 20180317142814.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2271-1 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2271-1 Chemical composition, larvicidal evaluation, and adult repellency of endemic Greek Thymus essential oils against the mosquito vector of West Nile virus [Elektronische Daten] [Danae Pitarokili, Antonios Michaelakis, George Koliopoulos, Athanassios Giatropoulos, Olga Tzakou] The volatile metabolites of Greek wild growing Thymus leucospermus and Thymus teucrioides subsp. candilicus were determined by gas chromatography and gas chromatography-mass spectrometry. The monoterpene hydrocarbon p-cymene (64.2%) dominated T. leucospermus oil, followed by γ-terpinene (7.9%), thymol (4.8%), and borneol (4.7%), whereas the most abundant constituents in T. teucrioides subsp. candilicus oil were p-cymene (25.5%), γ-terpinene (19.0%), thymol (18.8%), borneol (5.7%), and α-pinene (5.7%). The larvicidal and repellent activities of the analyzed essential oils were tested on Culex pipiens larvae and adults, respectively. Additionally, the main metabolite of the essential oils, p-cymene was tested against C. pipiens adults in order to define the affiliation between p-cymene and the repellent properties of the oil. The essential oils of T. leucospermus and T. teucrioides subsp. candilicus provided repellency 78.1% and 72.9%, respectively, statistically equal to the reference product icaridin. The compound p-cymene showed almost no repellent activity. The essential oil of T. leucospermus presented lower larvicidal activity (LC50 = 34.26mgl−1) against C. pipiens third-fourth instar larvae while T. teucrioides subsp. candilicus was the most active with an estimated LC50 value of 23.17mgl−1. Springer-Verlag, 2011 Pitarokili Danae Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimioupoli Zographou, 157 71, Athens, Greece aut Michaelakis Antonios Department of Entomology and Agricultural Zoology, Laboratory of Agricultural Entomology, Benaki Phytopathological Institute, 8 S. Delta Str., 14561, Kifissia, Athens, Greece aut Koliopoulos George Laboratory of Insecticides of Public Health Importance, Benaki Phytopathological Institute, 8 S. Delta Str, 14561, Kifissia, Athens, Greece aut Giatropoulos Athanassios Laboratory of Insecticides of Public Health Importance, Benaki Phytopathological Institute, 8 S. Delta Str, 14561, Kifissia, Athens, Greece aut Tzakou Olga Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimioupoli Zographou, 157 71, Athens, Greece aut Parasitology Research Springer-Verlag 109/2(2011-08-01), 425-430 0932-0113 109:2<425 2011 109 436 https://doi.org/10.1007/s00436-011-2271-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2271-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pitarokili Danae Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimioupoli Zographou, 157 71, Athens, Greece aut NATIONALLICENCE 700 1- Michaelakis Antonios Department of Entomology and Agricultural Zoology, Laboratory of Agricultural Entomology, Benaki Phytopathological Institute, 8 S. Delta Str., 14561, Kifissia, Athens, Greece aut NATIONALLICENCE 700 1- Koliopoulos George Laboratory of Insecticides of Public Health Importance, Benaki Phytopathological Institute, 8 S. Delta Str, 14561, Kifissia, Athens, Greece aut NATIONALLICENCE 700 1- Giatropoulos Athanassios Laboratory of Insecticides of Public Health Importance, Benaki Phytopathological Institute, 8 S. Delta Str, 14561, Kifissia, Athens, Greece aut NATIONALLICENCE 700 1- Tzakou Olga Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Panepistimioupoli Zographou, 157 71, Athens, Greece aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/2(2011-08-01), 425-430 0932-0113 109:2<425 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer