caa a22 4500 44534153X CHVBK 20180317142814.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2308-5 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2308-5 Gene expression during excystation of Cryptosporidium parvum oocysts [Elektronische Daten] [Mark Jenkins, Celia O'Brien, Kate Miska, Ryan Schwarz, Jeffrey Karns, Monica Santin, Ronald Fayer] The present study describes transcription of mRNA from genes encoding metabolic or structural proteins during excystation of Cryptosporidium parvum oocysts. RNA was harvested from C. parvum oocysts before excystation, and at 5, 10, and 15min during excystation. Subtractive cDNA libraries were prepared by using mRNA from non-excysted C. parvum oocysts to "subtract out” mRNA from excysting oocysts. The "subtracted” cDNA was used to prepare libraries enriched for transcripts possibly involved in excystation. From these libraries, over 1,000 expressed sequence tags (ESTs) were analyzed by DNA sequencing followed by BLAST-N and BLAST-X analysis. While several gene products involved in cell metabolism and cell signaling were consistently recovered, transcription levels, as reflected by the relative number of cDNA sequences (19.2% total), were highly up-regulated in genes coding for structural proteins such as Cp2, CpTSP, CpHC10, and CpSAg. Moreover, of the greater than 1,000 clones analyzed, a high percentage (12.3%) of ESTs detected in excysting oocysts were for hypothetical C. parvum proteins (CpHyP), whose functions are presently unknown. Springer-Verlag (outside the USA), 2011 Jenkins Mark Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut O'Brien Celia Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut Miska Kate Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut Schwarz Ryan Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut Karns Jeffrey Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut Santin Monica Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut Fayer Ronald Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut Parasitology Research Springer-Verlag 109/2(2011-08-01), 509-513 0932-0113 109:2<509 2011 109 436 https://doi.org/10.1007/s00436-011-2308-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2308-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jenkins Mark Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- O'Brien Celia Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Miska Kate Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Schwarz Ryan Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Karns Jeffrey Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Santin Monica Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut NATIONALLICENCE 700 1- Fayer Ronald Environmental Microbiology and Food Safety Laboratory, Agricultural Research Service, USDA, 20705, Beltsville, MD, USA aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/2(2011-08-01), 509-513 0932-0113 109:2<509 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer