caa a22 4500 445341556 CHVBK 20180317142814.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2262-2 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2262-2 Molecular cloning, expression, and characterization of cyclophilin A from Clonorchis sinensis [Elektronische Daten] [Weihua Wu, Jiajia Chen, Suxiang Zeng, Zhaoping Zhang, Wenjia Gan, Xinbing Yu, Xuchu Hu] This study described the recognization, cloning, and recombinant expression of cyclophilin A-like gene from Clonorchis sinensis adult complementary DNA library (CsCyPA) and its expression and secretion in adult. Western blotting demonstrated the recombinant CsCyPA could be recognized by sera of clonorchiasis patients and a sole protein of the same size in the excretory-secretory antigens of in vitro cultured adult could be recognized by antiserum raised against the recombinant CsCyPA. Immunohistochemistry demonstrated that the CsCyPA was secreted in scattered vesicles from subtegumental parenchyma cells to the surface of tegument and mainly released from the tegument. ELISA showed the serum levels of IgG against CsCyPA in clonorchiasis patients negatively correlated with worm loads. This study suggested that C. sinensis adult in biliary ducts could release CsCyPA without signal peptide through nonclassical secretory pathway into the liver and might play a role in inflammation and biliary epithelium proliferation and adenomatoid hyperplasia. Springer-Verlag, 2011 Wu Weihua Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut Chen Jiajia Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut Zeng Suxiang Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut Zhang Zhaoping Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut Gan Wenjia Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut Yu Xinbing Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut Hu Xuchu Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut Parasitology Research Springer-Verlag 109/2(2011-08-01), 345-351 0932-0113 109:2<345 2011 109 436 https://doi.org/10.1007/s00436-011-2262-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2262-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Weihua Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Chen Jiajia Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zeng Suxiang Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Zhaoping Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Gan Wenjia Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yu Xinbing Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Hu Xuchu Zhongshan School of Medicine, Department of Parasitology, Sun Yat-sen University, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/2(2011-08-01), 345-351 0932-0113 109:2<345 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer