caa a22 4500 445341602 CHVBK 20180317142815.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2276-9 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2276-9 Drug-induced apoptosis of Echinococcus granulosus protoscoleces [Elektronische Daten] [Hanhua Hu, Jinfeng Kang, Rong Chen, Wulamu Mamuti, Guizhen Wu, Wumei Yuan] Parasitic infection by Echinococcus granulosus in humans induces hydatidosis (echinococcosis), which is a zoonotic disease that seriously endangers public health. This study was to determine the status of cell apoptosis in the protoscoleces of E. granulosus, which were isolated from hydatid cysts in livers or lungs of sheep. Those protoscoleces were incubated with drugs (at the concentration of 1mmolL−1 H2O2 and 5mmolL−1dexamethasone) for 8h, the apoptosis were examined by transmission electron microscopy and TUNEL assay, the expression of caspase-1 and caspase-3 were detected by immunohistochemistry and caspase-3 activeity was detected by colorimetric assay. Our results have clearly demonstrated the presence of cell apoptosis in protoscoleces in the absence or presence of drug (H2O2, dexamethasone) treatment, but drug-induced apoptosis rate, caspase-1 and caspase-3 expression levels were higher than no-drug induce and caspase-3 activity were significantly increasing. We found H2O2 and dexamethasone can induce the cell apoptosis of protoscoleces. Our results implied the existence of a CED-3 like apoptosis gene in protoscolces and provide a rationale for further exploring the induction of apoptosis as non-surgical treatment method in treating this parasitic disease. Springer-Verlag, 2011 Hu Hanhua The Department of Electron Microscopy, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut Kang Jinfeng The Department of Parasitology, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut Chen Rong The Department of Electron Microscopy, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut Mamuti Wulamu The Department of Parasitology, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut Wu Guizhen The Department of Electron Microscopy, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut Yuan Wumei The Department of Electron Microscopy, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut Parasitology Research Springer-Verlag 109/2(2011-08-01), 453-459 0932-0113 109:2<453 2011 109 436 https://doi.org/10.1007/s00436-011-2276-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2276-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Hanhua The Department of Electron Microscopy, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut NATIONALLICENCE 700 1- Kang Jinfeng The Department of Parasitology, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut NATIONALLICENCE 700 1- Chen Rong The Department of Electron Microscopy, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut NATIONALLICENCE 700 1- Mamuti Wulamu The Department of Parasitology, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut NATIONALLICENCE 700 1- Wu Guizhen The Department of Electron Microscopy, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut NATIONALLICENCE 700 1- Yuan Wumei The Department of Electron Microscopy, Basical Medicine College, Xinjiang Medical University, 830011, Urumqi, Xinjiang, China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/2(2011-08-01), 453-459 0932-0113 109:2<453 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer