caa a22 4500 445341653 CHVBK 20180317142815.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s00436-011-2279-6 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2279-6 Induction of proinflammatory cytokines and nitric oxide by Trypanosoma cruzi in renal cells [Elektronische Daten] [Gabriel de Oliveira, Nobuko Yoshida, Elisa Higa, Sérgio Shenkman, Monique Alves, Daniela Staquicini, Cynthia Cascabulho, Nestor Schor] Chagas disease is typically associated with cardiac involvement. During the acute phase of murine infection with Trypanosoma cruzi, severe acute myocarditis can develop. Prior to cardiac alteration, however, infected mice present with renal inflammatory infiltration causing acute kidney injury due to an ischemia/reperfusion lesion. Thus, the present study was undertaken in order to evaluate whether the parasites or some of their components would directly affect renal cells. As such, this study employed kidney cell lines (mesangial, epithelial, and proximal tubular) that mimic different regions of the renal system. Mesangial cells are more resistant to infection, showing reduced parasite internalization relative to epithelial and proximal tubular cells. Upon infection, mesangial cells produced more nitric oxide, tumor factor necrosis-α, and interferon-γ and showed decreased viability when compared to the other cell lines. These results indicate that the resistance of mesangial cells to infection may be related to the increased expression of nitric oxide and proinflammatory cytokines. Conversely, the high levels of nitric oxide produced by these cells caused impairment of cell integrity and viability. Higher nitric oxide concentrations promote cellular injury and can be involved in the genesis of ischemia/reperfusion lesions in acute kidney injury. Springer-Verlag, 2011 de Oliveira Gabriel Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, RJ, Brazil aut Yoshida Nobuko Dept. de Microbiologia, Imunologia e Parasitologia, São Paulo, SP, Brazil aut Higa Elisa Dept. de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut Shenkman Sérgio Dept. de Microbiologia, Imunologia e Parasitologia, São Paulo, SP, Brazil aut Alves Monique Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, RJ, Brazil aut Staquicini Daniela Dept. de Microbiologia, Imunologia e Parasitologia, São Paulo, SP, Brazil aut Cascabulho Cynthia Laboratório de Inovações em Terapias, Ensino e Bioprodutos, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ, Brazil aut Schor Nestor Dept. de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut Parasitology Research Springer-Verlag 109/2(2011-08-01), 483-491 0932-0113 109:2<483 2011 109 436 https://doi.org/10.1007/s00436-011-2279-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2279-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Oliveira Gabriel Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 700 1- Yoshida Nobuko Dept. de Microbiologia, Imunologia e Parasitologia, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Higa Elisa Dept. de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Shenkman Sérgio Dept. de Microbiologia, Imunologia e Parasitologia, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Alves Monique Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos, 21045-900, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 700 1- Staquicini Daniela Dept. de Microbiologia, Imunologia e Parasitologia, São Paulo, SP, Brazil aut NATIONALLICENCE 700 1- Cascabulho Cynthia Laboratório de Inovações em Terapias, Ensino e Bioprodutos, Instituto Oswaldo Cruz/FIOCRUZ, Rio de Janeiro, RJ, Brazil aut NATIONALLICENCE 700 1- Schor Nestor Dept. de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 109/2(2011-08-01), 483-491 0932-0113 109:2<483 2011 109 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer