caa a22 4500 44534170X CHVBK 20180317142815.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00436-010-2059-8 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2059-8 Genetic relationships within the Opisthorchis viverrini species complex with specific analysis of O. viverrini from Savannakhet, Lao PDR by multilocus enzyme electrophoresis [Elektronische Daten] [Nadda Kiatsopit, Paiboon Sithithaworn, Jiraporn Sithithaworn, Thidarut Boonmars, Smarn Tesana, Opal Pitaksakulrat, Weerachai Saijuntha, Trevor Petney, Ross Andrews] Recent systematic studies of Opisthorchis viverrini based on multilocus enzyme electrophoresis (MEE) have shown that there are at least five genetic groups and possibly two cryptic species occurring in Thailand and Lao PDR each associated with a specific wetland system. A study based on mitochondrial DNA (mtDNA) gene analyses of an O. viverrini population from Savannakhet (SV, Lao PDR) clustered with several isolates from Thailand and Lao PDR although they originated from different river wetland systems. We used MEE to re-examine whether O. viverrini from SV was similar genetically to isolates from Thailand and Lao PDR. The allelic profiles of O. viverrini from SV and five different wetlands representing defined genetic groups of O. viverrini were recorded at 24 enzyme loci as opposed to only two loci of mtDNA. Contrary to previous studies, O. viverrini from SV was found to have fixed genetic differences at six to eight of the 24 loci examined (24.50-35.42%). Allelic data indicated that O. viverrini from SV differed from isolates in the Nam Ngum River wetland in Lao PDR (29.33% fixed genetic differences) and clustered with O. viverrini from Nakhon Phanom and Sakon Nakhon within the Songkram River wetland in Thailand but had fixed genetic differences from these at 24.5% of loci examined. Our data confirm the association between genetic groups of O. viverrini and specific wetland systems, and raise important questions regarding the significance of the genetic differences and relationships of O. viverrini from these wetlands. Springer-Verlag, 2010 Kiatsopit Nadda Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Sithithaworn Paiboon Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Sithithaworn Jiraporn Faculty of Associated Medical Sciences, Khon Kaen University, 40002, Khon Kaen, Thailand aut Boonmars Thidarut Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Tesana Smarn Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Pitaksakulrat Opal Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Saijuntha Weerachai Walai Rukhavej Botanical Research Institute, Mahasarakham University, 44150, Mahasarakham, Thailand aut Petney Trevor Institute of Zoology 1: Ecology and Parasitology, University of Karlsruhe, Kornblumen Strasse 13, Karlsruhe, Germany aut Andrews Ross Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Parasitology Research Springer-Verlag 108/1(2011-01-01), 211-217 0932-0113 108:1<211 2011 108 436 https://doi.org/10.1007/s00436-010-2059-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2059-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kiatsopit Nadda Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Sithithaworn Paiboon Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Sithithaworn Jiraporn Faculty of Associated Medical Sciences, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Boonmars Thidarut Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Tesana Smarn Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Pitaksakulrat Opal Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Saijuntha Weerachai Walai Rukhavej Botanical Research Institute, Mahasarakham University, 44150, Mahasarakham, Thailand aut NATIONALLICENCE 700 1- Petney Trevor Institute of Zoology 1: Ecology and Parasitology, University of Karlsruhe, Kornblumen Strasse 13, Karlsruhe, Germany aut NATIONALLICENCE 700 1- Andrews Ross Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/1(2011-01-01), 211-217 0932-0113 108:1<211 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer