caa a22 4500 445341769 CHVBK 20180317142815.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00436-010-2049-x doi (NATIONALLICENCE)springer-10.1007/s00436-010-2049-x Development of cellular immune response of mice to infection with low doses of Trichinella spiralis , Trichinella britovi and Trichinella pseudospiralis larvae [Elektronische Daten] [Emília Dvorožňáková, Zuzana Hurníková, Marta Kołodziej-Sobocińska] The murine cellular immune response to the infection with ten larvae of encapsulating (Trichinella spiralis, Trichinella britovi) and non-encapsulating species (Trichinella pseudospiralis) was studied. Both T. spiralis and T. britovi stimulated the proliferation of splenic T and B lymphocytes during the intestinal phase of infection, but T. spiralis activated the proliferative response also at the muscle phase, particularly in B cells. Non-encapsulating T. pseudospiralis stimulated the proliferation of T and B cells only on day10 post-infection (p.i.) and later at the muscle phase. The numbers of splenic CD4 and CD8 T cells of T. spiralis infected mice were significantly increased till day10 p.i., i.e., at the intestinal phase, and then at the late muscle phase, on day60 p.i. T. britovi infection increased the CD4 and CD8 T cell numbers only on day30 p.i. Decreased numbers of CD4 and CD8 T cells after T. pseudospiralis infection suggest a suppression of cellular immunity. Both encapsulating Trichinella species induced the Th2 response (cytokines interleukin-5 (IL-5) and interleukin-10) at the intestinal phase and the Th2 dominant response at the advanced muscle phase. Interferon-γ (IFN-γ) production (Th1 type) started to increase with migrating newborn larvae from day15 p.i. till the end of the experiment. IL-5 production was suppressed during the intestinal phase of T. pseudospiralis infection. The immune response to T. pseudospiralis was directed more to the Th1 response at the muscle phase, the high IFN-γ production was found on day10 p.i. and it peaked on days45 and 60 p.i. Springer-Verlag, 2010 Dvorožňáková Emília Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic aut Hurníková Zuzana Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic aut Kołodziej-Sobocińska Marta Institute of Parasitology of the Polish Academy of Sciences, Twarda str 51/55, 00 818, Warsaw, Poland aut Parasitology Research Springer-Verlag 108/1(2011-01-01), 169-176 0932-0113 108:1<169 2011 108 436 https://doi.org/10.1007/s00436-010-2049-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2049-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dvorožňáková Emília Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic aut NATIONALLICENCE 700 1- Hurníková Zuzana Parasitological Institute of the Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic aut NATIONALLICENCE 700 1- Kołodziej-Sobocińska Marta Institute of Parasitology of the Polish Academy of Sciences, Twarda str 51/55, 00 818, Warsaw, Poland aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/1(2011-01-01), 169-176 0932-0113 108:1<169 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer