caa a22 4500 445341777 CHVBK 20180317142815.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00436-010-2046-0 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2046-0 Sequential recognition of antigenic markers of Toxoplasma gondii tachyzoite by pooled sera of mice with experimental toxoplasmosis [Elektronische Daten] [Fayza Habib, Nehad Ali, Azza El-kadery, Salwa Soffar, Mona Abdel-Razek] Accurate diagnosis of maternal toxoplasmosis can enhance the success of medical treatment and prevent congenital transmission. The current diagnostic methods have many limits, and they poorly differentiate between recent and latent infections. The present work was conducted to record the sequential recognition of antigenic markers of both Toxoplasma tachyzoites whole extract and glycosylinositolphospholipids (GIPLs)-enriched fraction by specific IgG and IgM, respectively, by immunoblotting analysis of the antigens against daily pooled serum samples from mice with experimentally induced recent and latent toxoplasmosis. IgG avidity immunoblotting was tested by using a wash with 6M urea solution as antigen-antibody disrupting agent. Band of 10kDa reacted exclusively with low-avidity IgG in pooled sera of mice with recent infection. Band of 39kDa was a good marker for the infection; reacting with both low-avidity IgG in recent infection and with high-avidity IgG in latent one. Bands of 15, 23, 30, 60, 66, and 97kDa reacted with variable avidity in both phases of infection. Two antigenic bands were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the GIPLs-enriched fraction of tachyzoite, the 14- and 30-kDa band. The 14-kDa band was recognized by IgM in pooled serum samples of recently infected mice only, while the 30-kDa band was recognized by serum samples of both recent and latent phases of infections. The study highlights the value of avidity immunoblotting assay to discriminate between recent and latent experimental toxoplasmosis. Further study must be carried on human to evaluate the values of the used technique. Springer-Verlag, 2010 Habib Fayza Parasitology Department, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut Ali Nehad Parasitology Department, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut El-kadery Azza Parasitology Department, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut Soffar Salwa Parasitology Department, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut Abdel-Razek Mona Parasitology Department, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut Parasitology Research Springer-Verlag 108/1(2011-01-01), 151-160 0932-0113 108:1<151 2011 108 436 https://doi.org/10.1007/s00436-010-2046-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2046-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Habib Fayza Parasitology Department, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Ali Nehad Parasitology Department, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- El-kadery Azza Parasitology Department, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Soffar Salwa Parasitology Department, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 700 1- Abdel-Razek Mona Parasitology Department, Faculty of Medicine, Ain Shams University, 11566, Cairo, Egypt aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/1(2011-01-01), 151-160 0932-0113 108:1<151 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer