caa a22 4500 445341793 CHVBK 20180317142815.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00436-010-2050-4 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2050-4 Cloning and expression of 21.1-kDa tegumental protein of Clonorchis sinensis and human antibody response to it as a trematode-nematode pan-specific serodiagnosis antigen [Elektronische Daten] [Jiajia Chen, Hongxu Xu, Zhaoping Zhang, Suxiang Zeng, Wenjia Gan, Xinbing Yu, Xuchu Hu] A complete cDNA encoding a 21.1-kDa tegumental protein (CsTP21.1) was recognized from Clonorchis sinensis adult full-length cDNA plasmid library by bioinformatics analysis. Recombinant CsTP21.1 was highly expressed in Escherichia coli, purified by affinity chromatography, and identified by Western blotting. Immunohistochemistry demonstrated that CsTP21.1 is localized in the tegument of the adult worm. The rCsTP21.1-specific IgG1, IgG2, and IgG4 subclasses could be detected in the sera of clonorchiasis patients by ELISA, but their sensitivity was much lower than that of total IgG. The sensitivity and specificity of IgG in 66 serum samples of clonorchiasis patients were 100% and 95.5%, and the sensitivity was independent of worm loads; the cross-reaction rates in 86, 24, and 31 serum samples from patients infected with Fasciola hepatica, Schistosoma japonicum, and nematode were 98.8%, 83.3%, 93.3%, respectively, whereas no cross-reactions with Toxoplasma gondii and sparganum. This study demonstrated that CsTP21.1 is a trematode-nematode pan-specific antigen that is valuable in the development of a universal immunodiagnostic kit for human trematode and nematode infections. Springer-Verlag, 2010 Chen Jiajia Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut Xu Hongxu Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China aut Zhang Zhaoping Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut Zeng Suxiang Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut Gan Wenjia Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut Yu Xinbing Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut Hu Xuchu Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut Parasitology Research Springer-Verlag 108/1(2011-01-01), 161-168 0932-0113 108:1<161 2011 108 436 https://doi.org/10.1007/s00436-010-2050-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2050-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Jiajia Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 700 1- Xu Hongxu Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 700 1- Zhang Zhaoping Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 700 1- Zeng Suxiang Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 700 1- Gan Wenjia Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 700 1- Yu Xinbing Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 700 1- Hu Xuchu Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/1(2011-01-01), 161-168 0932-0113 108:1<161 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer