caa a22 4500 445341858 CHVBK 20180317142815.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00436-010-2040-6 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2040-6 Construction, purification, and evaluation of multivalent DNA vaccine against Schistosoma japonicum [Elektronische Daten] [Lu Zhu, Hai-Feng Liu, Ming-Bo Lu, Quan-Ke Long, You-En Shi, Long-Jiang Yu] DNA vaccine encoding a multivalent antigen is a novel approach of protective immunization. Four Schistosoma japonicum candidate antigen genes, glyceraldehyde-3-phosphate dehydrogenase (SjGAPDH), 23kDa transmembrane protein (Sj23), 14kDa fatty-acid binding protein (SjFABP) and 26kDa glutathione-S-transferase (Sj26), are recombined into two pieces of fusion genes SjFABP.Sj23 and Sj26.SjGAPDH, respectively. Tetravalent DNA vaccine pVIVO2-SjFABP.Sj23/Sj26.SjGAPDH is constructed by co-expressing these two fusion genes. The super-coiled DNA vaccines for large-scale clinic application were purified by sequential chromatographies including group separation chromatography and affinitive chromatographies. The purified DNA vaccines were evaluated for in vivo and in vitro transfection assay. The immunoprotective properties of the different kinds of constructed DNA vaccines were appraised by pharmacological trials. The pharmacological trials results showed that tetravalent DNA vaccine has higher protective efficiency than other tested DNA vaccines. Springer-Verlag, 2010 Zhu Lu College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China aut Liu Hai-Feng College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China aut Lu Ming-Bo College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China aut Long Quan-Ke College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China aut Shi You-En Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China aut Yu Long-Jiang College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China aut Parasitology Research Springer-Verlag 108/1(2011-01-01), 115-121 0932-0113 108:1<115 2011 108 436 https://doi.org/10.1007/s00436-010-2040-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2040-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhu Lu College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Liu Hai-Feng College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Lu Ming-Bo College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Long Quan-Ke College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Shi You-En Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China aut NATIONALLICENCE 700 1- Yu Long-Jiang College of Life Science and Technology, Huazhong University of Science and Technology, 430074, Wuhan, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/1(2011-01-01), 115-121 0932-0113 108:1<115 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer