caa a22 4500 445341890 CHVBK 20180317142816.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00436-010-2032-6 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2032-6 Immune detection of acetylcholinesterase in subcellular compartments of Trypanosoma evansi [Elektronische Daten] [Alfredo Mijares, Juan Concepción, Jose Vielma, Ramon Portillo] Trypanosoma evansi is a worldwide distributed hemoparasite with a strong economic impact in veterinary activities. Despite widespread knowledge about the etiology of the disease caused by T. evansi, there are few detailed studies about the metabolism of this parasite. The aim of this study was to determine the presence of Acetylcholinesterase (AChE) in T. evansi through a strategy of subcellular localization and confocal microscopy. The localization of the AChE by differential and isopycnic centrifugation strategy showed that this enzyme has a predominant localization in the glycosome, similar to hexokinase, and it is not present in either the cytosol or the plasma membrane. This study shows novel data that help to understand the non-neuronal role of AChE in the Trypanosomatidae family. Springer-Verlag, 2010 Mijares Alfredo Centro de Biofísica y Bioquímica, Laboratorio de Fisiología de Parásitos, Instituto Venezolano de Investigaciones Científicas, A.P. 20632, 1020A, Caracas, Venezuela aut Concepción Juan Facultad de Ciencias, Laboratorio de Enzimología de Parásitos, Universidad de Los Andes, Merida, Venezuela aut Vielma Jose Facultad de Ciencias, Laboratorio de Enzimología de Parásitos, Universidad de Los Andes, Merida, Venezuela aut Portillo Ramon Centro de Biofísica y Bioquímica, Laboratorio de Fisiología de Parásitos, Instituto Venezolano de Investigaciones Científicas, A.P. 20632, 1020A, Caracas, Venezuela aut Parasitology Research Springer-Verlag 108/1(2011-01-01), 1-5 0932-0113 108:1<1 2011 108 436 https://doi.org/10.1007/s00436-010-2032-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2032-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mijares Alfredo Centro de Biofísica y Bioquímica, Laboratorio de Fisiología de Parásitos, Instituto Venezolano de Investigaciones Científicas, A.P. 20632, 1020A, Caracas, Venezuela aut NATIONALLICENCE 700 1- Concepción Juan Facultad de Ciencias, Laboratorio de Enzimología de Parásitos, Universidad de Los Andes, Merida, Venezuela aut NATIONALLICENCE 700 1- Vielma Jose Facultad de Ciencias, Laboratorio de Enzimología de Parásitos, Universidad de Los Andes, Merida, Venezuela aut NATIONALLICENCE 700 1- Portillo Ramon Centro de Biofísica y Bioquímica, Laboratorio de Fisiología de Parásitos, Instituto Venezolano de Investigaciones Científicas, A.P. 20632, 1020A, Caracas, Venezuela aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/1(2011-01-01), 1-5 0932-0113 108:1<1 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer