caa a22 4500 445341904 CHVBK 20180317142816.0 cr unu---uuuuu 170323e20110101xx s 000 0 eng 10.1007/s00436-010-2058-9 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2058-9 Anthelmintic activity of the crude extracts, fractions, and osthole from Radix angelicae pubescentis against Dactylogyrus intermedius in goldfish ( Carassius auratus ) in vivo [Elektronische Daten] [Kai-yu Wang, Lu Yao, Yong-hua Du, Jia-bing Xie, Jin-lu Huang, Zhong-qiong Yin] A bioassay-guided fractionation was performed to evaluate the anthelmintic activity of the crude extract fractions and osthole from Radix angelicae pubescentis against Dactylogyrus intermedius in goldfish (Carassius auratus) in vivo. Among four extracts (petroleum ether, ethyl acetate, acetone, and ethanol), only ethanol extract exhibited the best anthelmintic efficacy with 100% mortality of Dactylogyrus and no death of fish at the optimal anthelminthic concentration of 120mg/L. Therefore, ethanol extract was subjected to column chromatography to obtain sixteen fractions. The activity was found in fraction F with 100% mortality of Dactylogyrus and no toxicity to fish at dose of 2.0mg/L. A white crystal was isolated from fraction F and identified as osthole which exhibited the optimal activity with 100% mortality of Dactylogyrus at 1.6mg/L had and no toxicity to fish at dose up to 6.2mg/L. This is the first report on the isolation and identification of anthelmintic active compound from R. angelicae pubescentis against D. intermedius in goldfish (C. auratus) in vivo. Springer-Verlag, 2010 Wang Kai-yu Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut Yao Lu Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut Du Yong-hua Key Laboratory of Fermentation Resource and Application of Institutes of Higher Learning in Sichuan, Yibin University, 644007, Yibin, China aut Xie Jia-bing Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut Huang Jin-lu Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut Yin Zhong-qiong Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut Parasitology Research Springer-Verlag 108/1(2011-01-01), 195-200 0932-0113 108:1<195 2011 108 436 https://doi.org/10.1007/s00436-010-2058-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2058-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Kai-yu Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut NATIONALLICENCE 700 1- Yao Lu Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut NATIONALLICENCE 700 1- Du Yong-hua Key Laboratory of Fermentation Resource and Application of Institutes of Higher Learning in Sichuan, Yibin University, 644007, Yibin, China aut NATIONALLICENCE 700 1- Xie Jia-bing Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut NATIONALLICENCE 700 1- Huang Jin-lu Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut NATIONALLICENCE 700 1- Yin Zhong-qiong Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, 625014, Ya'an, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/1(2011-01-01), 195-200 0932-0113 108:1<195 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer