caa a22 4500 445341955 CHVBK 20180317142816.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00436-010-2198-y doi (NATIONALLICENCE)springer-10.1007/s00436-010-2198-y Improving the detection limit of quantitative diagnosis of anti- S. haematobium antibodies using Falcon Assay Screening Test (FAST) ELISA by developing a new standard curve [Elektronische Daten] [Mohamed Abdel-Fattah, Maged Al-Sherbiny, Ahmed Osman, Ragia Charmy, Victor Tsang] Immunodiagnosis of schistosomiasis are currently based on parasitological examinations of stool and urine for egg detection, which is laborious and lacks sensitivity. There are many assays that detect the anti-schistosomal antibodies in patient sera. One of these assays is the Falcon assay screening test (FAST) ELISA that uses adult worm microsomal antigen for Schistosoma haematobium and Schistosoma mansoni, HAMA, MAMA antigen, respectively. This assay depends on quantitative detection of anti-schistosome antibodies, using a standard curve, but the detection limit of FAST-HAMA assay is low due to the small range of the standard curve. In our study, a new wide range (0-80μl/μl) of standard curve for FAST-HAMA assay was constructed, and the cut-off value of the assay, using the new curve, was determined to be 1.2 units/μl. Screening of 41 S. haematobium-infected sera with FAST-HAMA, using the new constructed curve, showed a sensitivity of 95%. The purity of HAMA antigen and highly specific Abs for S. haematobium lends the FAST-HAMA with the new constructed wide range standard curve as a diagnostic assay with high detection limit for S. haematobium infection. Springer-Verlag, 2010 Abdel-Fattah Mohamed R&D Department, Holding Company for Biological Products and Vaccines (VACSERA), 51 Wezaret El-Zeraa St., Agouza, Giza, Egypt aut Al-Sherbiny Maged Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt aut Osman Ahmed Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt aut Charmy Ragia Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt aut Tsang Victor Center for Disease Control and Prevention, Atlanta, GA, USA aut Parasitology Research Springer-Verlag 108/6(2011-06-01), 1457-1463 0932-0113 108:6<1457 2011 108 436 https://doi.org/10.1007/s00436-010-2198-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2198-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abdel-Fattah Mohamed R&D Department, Holding Company for Biological Products and Vaccines (VACSERA), 51 Wezaret El-Zeraa St., Agouza, Giza, Egypt aut NATIONALLICENCE 700 1- Al-Sherbiny Maged Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt aut NATIONALLICENCE 700 1- Osman Ahmed Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt aut NATIONALLICENCE 700 1- Charmy Ragia Department of Zoology, Faculty of Science, Cairo University, Giza, Egypt aut NATIONALLICENCE 700 1- Tsang Victor Center for Disease Control and Prevention, Atlanta, GA, USA aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/6(2011-06-01), 1457-1463 0932-0113 108:6<1457 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer