caa a22 4500 445342021 CHVBK 20180317142816.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00436-010-2183-5 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2183-5 Qualitative and quantitative immunohistochemical evaluation of iNOS expression in the spleen of dogs naturally infected with Leishmania chagasi [Elektronische Daten] [Fernando dos Santos, Paula Vieira, Rodrigo Correa-Oliveira, Rodolfo Giunchetti, Claudia Carneiro, Alexandre Reis, Luiz Malaquias] Nitric oxide (NO), the product of the nitric oxide synthase enzymes has been detected in Leishmania-infected animals. Besides its role on the immunity to infection, the role of NO and the inducible nitric oxide synthase (iNOS) in the pathogenesis of canine visceral leishmaniasis (CVL) is not well understood. This study aimed at evaluating immunohistochemically the iNOS expression in the spleen of dogs naturally infected (ID) with Leishmania (L.) chagasi compared with non-infected dogs (NID). The ID was grouped according to the clinical form and the parasite load. Symptomatic dogs (SD) presented higher parasite load in relation to oligosymptomatic (OD) and asymptomatic (AD). The qualitative expression of iNOS was observed only in ID. SD presented strong and prominent labeling of iNOS, followed by OD and AD. Quantitatively, the results showed that the median expression of iNOS was higher in SD and OD compared to NID. Also, dog spleens with high parasitism load showed marked iNOS expression. Taken together, the results suggest that the expression of iNOS in the spleen of infected dogs with CVL was associated with clinical worsening of the disease and with high parasitism. Springer-Verlag, 2010 dos Santos Fernando Universidade Presidente Antonio Carlos, Teófilo Otoni, MG, Brazil aut Vieira Paula Laboratório de Imunopatologia, NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil aut Correa-Oliveira Rodrigo Laboratório de Imunologia Celular e Molecular, Instituto René Rachou—FIOCRUZ, Belo Horizonte, MG, Brazil aut Giunchetti Rodolfo Laboratório de Imunopatologia, NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil aut Carneiro Claudia Laboratório de Imunopatologia, NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil aut Reis Alexandre Laboratório de Imunopatologia, NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil aut Malaquias Luiz Laboratório de Microbiologia e Imunologia Básicas, Universidade Federal de Alfenas, Alfenas, MG, Brazil aut Parasitology Research Springer-Verlag 108/6(2011-06-01), 1397-1403 0932-0113 108:6<1397 2011 108 436 https://doi.org/10.1007/s00436-010-2183-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2183-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- dos Santos Fernando Universidade Presidente Antonio Carlos, Teófilo Otoni, MG, Brazil aut NATIONALLICENCE 700 1- Vieira Paula Laboratório de Imunopatologia, NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil aut NATIONALLICENCE 700 1- Correa-Oliveira Rodrigo Laboratório de Imunologia Celular e Molecular, Instituto René Rachou—FIOCRUZ, Belo Horizonte, MG, Brazil aut NATIONALLICENCE 700 1- Giunchetti Rodolfo Laboratório de Imunopatologia, NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil aut NATIONALLICENCE 700 1- Carneiro Claudia Laboratório de Imunopatologia, NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil aut NATIONALLICENCE 700 1- Reis Alexandre Laboratório de Imunopatologia, NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil aut NATIONALLICENCE 700 1- Malaquias Luiz Laboratório de Microbiologia e Imunologia Básicas, Universidade Federal de Alfenas, Alfenas, MG, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/6(2011-06-01), 1397-1403 0932-0113 108:6<1397 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer