caa a22 4500 445342048 CHVBK 20180317142816.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00436-010-2202-6 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2202-6 First detection and genotyping of Encephalitozoon cuniculi in a new host species, gyrfalcon ( Falco rusticolus) [Elektronische Daten] [Beata Malcekova, Alexandra Valencakova, Lenka Luptakova, Ladislav Molnar, Petra Ravaszova, Frantisek Novotny] Recently, the pathogenic species of microsporidia of the genus Encephalitozoon have been detected increasingly, also in representatives of the Aves class. Our study presents laboratory proof of Encephalitozoon cuniculi (E. cuniculi) genotype II in a new host, gyrfalcon (Falco rusticolus), with suspect microsporidiosis. E. cuniculi is an obligate intracellular microsporidian parasite that infects a wide range of mammalian hosts, including humans. Characterization of the internal transcribed spacer of the rRNA gene has identified three genotypes of E. cuniculi based on the number of 5′-GTTT-3′ repeats present: a genotype I from rabbits and mice, containing three repeats; a genotype II from mice and dogs, containing two repeats; and a genotype III from dogs and fox, containing four repeats. Samples of faeces from 30 gyrfalcons were examined for the presence of microsporidia spores, using microscopical, molecular methods and sequencing. Microscopic analysis showed presence of brightly fluorescing oval shapes of size 1.5 × 3μm, characteristic of the strain Microsporidia in five samples. The PCR method, using species non-specific (530F/580R) and species-specific (ECUNR/ECUNF) primers, proved the presence of E. cuniculi spores in two samples. After sequencing were confirmed, E. cuniculi genotype II which implies new host species for this parasite. Springer-Verlag, 2010 Malcekova Beata Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut Valencakova Alexandra Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut Luptakova Lenka Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut Molnar Ladislav Avian and Exotic Clinic, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut Ravaszova Petra Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut Novotny Frantisek Clinic of Horses, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut Parasitology Research Springer-Verlag 108/6(2011-06-01), 1479-1482 0932-0113 108:6<1479 2011 108 436 https://doi.org/10.1007/s00436-010-2202-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2202-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Malcekova Beata Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut NATIONALLICENCE 700 1- Valencakova Alexandra Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut NATIONALLICENCE 700 1- Luptakova Lenka Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut NATIONALLICENCE 700 1- Molnar Ladislav Avian and Exotic Clinic, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut NATIONALLICENCE 700 1- Ravaszova Petra Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut NATIONALLICENCE 700 1- Novotny Frantisek Clinic of Horses, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/6(2011-06-01), 1479-1482 0932-0113 108:6<1479 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer