caa a22 4500 445342080 CHVBK 20180317142816.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00436-010-2210-6 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2210-6 Biochemical evidence of efficacy of potash alum for the control of dengue vector Aedes aegypti (Linnaeus) [Elektronische Daten] [Shabad Preet, A. Sneha] Aedes aegypti is the primary vector of dengue, yellow fever and chikungunya in India and other South East Asian countries, and novel insecticides for vector control are urgently needed. In the present investigation, efficacy of potash alum, a traditionally known double salt in Indian and Chinese medicine system, was tested against the larvae of dengue vector, A. aegypti. LC50, LC90 and LC99 values were recorded for various instar larvae where I instar larvae were found to be the most susceptible and IV instar larvae as the least susceptible one. The LC50 values of crude and standard potash alum of various instar larvae ranged between 15.29 and 48.53ppm and 20.50-65.10ppm, respectively. Biochemical changes were also evidenced in IV instar A. aegypti larvae following a sublethal exposure for 24h in the levels of various nutrient reserves and primary metabolites such as sugar, glycogen, lipids and proteins suggesting possible mode of action responsible for larval mortality. Sugar and glycogen concentrations were measured as 24.6 and 10.67μg per five larvae in controls which were significantly (p < 0.05) reduced by 32.11-93.98% and 39.26-94.47%, respectively, in larvae treated with crude alum. In controls, protein and lipid content were recorded as 210.74 and 94.71μg per five larvae which dropped up to 26.53% and 25.5%, respectively, in larvae following treatment with crude alum. Moreover, drastic changes were also recorded for DNA content with 25.39-44.17% decrease in crude alum-treated larvae. It is evident from these results that potash alum, a fairly cheaper and readily available ecofriendly compound could be recommended as a potential chemical larvicide against dengue vector at mosquito breeding sites in the vicinity of human dwellings. Springer-Verlag, 2010 Preet Shabad Department of Zoology, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, 282110, Agra, India aut Sneha A. Department of Zoology, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, 282110, Agra, India aut Parasitology Research Springer-Verlag 108/6(2011-06-01), 1533-1539 0932-0113 108:6<1533 2011 108 436 https://doi.org/10.1007/s00436-010-2210-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2210-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Preet Shabad Department of Zoology, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, 282110, Agra, India aut NATIONALLICENCE 700 1- Sneha A. Department of Zoology, Faculty of Science, Dayalbagh Educational Institute, Dayalbagh, 282110, Agra, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/6(2011-06-01), 1533-1539 0932-0113 108:6<1533 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer