caa a22 4500 445342153 CHVBK 20180317142816.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00436-010-2206-2 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2206-2 Serodiagnosis of experimental sparganum infections of mice and human sparganosis by ELISA using ES antigens of Spirometra mansoni spargana [Elektronische Daten] [Jing Cui, Nan Li, Zhong Wang, Peng Jiang, Xi Lin] We conducted a study of serodiagnosis of experimental sparganum infections of mice and human sparganosis by enzyme-linked immunosorbent assay (ELISA) using excretory-secretory (ES) antigens of Spirometra mansoni spargana and compared the sensitivity and specificity of crude and ES antigens for detecting the specific anti-sparganum IgG antibodies. By crude antigen ELISA and ES antigen ELISA, anti-sparganum IgG was detected in all of 30 serum samples of the infected mice; no cross-reactions were observed in serum samples of the mice infected with Trichinella spiralis, Schistosoma japanicum, Toxoplasma gondii, and normal mice. Anti-sparganum IgG was detected by ES antigen ELISA in sera of mice infected with one, two, four, six, and eight spargana at 3weeks post-infection (wpi), with a detection rate of 100%, and lasted to 18wpi when the experiment was ended. The difference in anti-sparganum antibody levels among five groups of the infected mice was statistically significant (F = 245.296, p < 0.05); the antibody levels were correlated with infecting doses of spargana (r = 0.323, p < 0.05). The sensitivity of both ELISA in detecting the serum samples of patients with sparganosis was 100% (20/20), but 96.72% (59/61) of specificity of ES antigen ELISA in detecting serum samples of patients with cysticercosis, echinococcosis, paragonimiosis, clonorchiosis, and schistosomiasis, and healthy persons was significantly greater than 72.13% (44/61) of crude antigen ELISA (χ 2 = 14.027, p < 0.05). Our finding indicates that ELISA using ES antigens of S. mansoni spargana may be applied to the specific early serodiagnosis of sparganosis. Springer-Verlag, 2010 Cui Jing Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut Li Nan Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut Wang Zhong Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut Jiang Peng Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut Lin Xi Department of Helminthiasis, Henan Center for Disease Control and Prevention, Zhengzhou, People's Republic of China aut Parasitology Research Springer-Verlag 108/6(2011-06-01), 1551-1556 0932-0113 108:6<1551 2011 108 436 https://doi.org/10.1007/s00436-010-2206-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2206-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cui Jing Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Li Nan Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zhong Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Peng Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Lin Xi Department of Helminthiasis, Henan Center for Disease Control and Prevention, Zhengzhou, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/6(2011-06-01), 1551-1556 0932-0113 108:6<1551 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer