caa a22 4500 445342161 CHVBK 20180317142816.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00436-011-2363-y doi (NATIONALLICENCE)springer-10.1007/s00436-011-2363-y In vitro antiplasmodial activity and cytotoxicity of crude extracts and compounds from the stem bark of Kigelia africana (Lam.) Benth (Bignoniaceae) [Elektronische Daten] [Denis Zofou, Archile Kengne, Mathieu Tene, Moses Ngemenya, Pierre Tane, Vincent Titanji] In order to assess the potential of the stem bark of Kigelia africana (Lam.) Benth as source of new anti-malarial leads, n-hexane and ethyl acetate (EtOAc) extracts and four compounds isolated from the stem bark were screened in vitro against the chloroquine-resistant W-2 and two field isolates of Plasmodium falciparum using lactate dehydrogenase assay. The products were also tested for their cytotoxicity on LLC/MK2 monkey kidney cells. The EtOAc extract exhibited a significant antiplasmodial activity (IC50 = 11.15μg/mL on W-2; 3.91 and 4.74μg/mL on field CAM10 and SHF4 isolates, respectively), whereas the n-hexane fraction showed a weak activity (IC50 = 73.78μg/mL on W-2 and 21.85μg/mL on SHF4). Three out of the four compounds showed good activity against all the three different parasite strains (IC50 < 5μM). Specicoside exhibited the highest activity on W-2 (IC50 = 1.54μM) followed by 2β, 3β, 19α-trihydroxy-urs-12-en-28-oic acid (IC50 = 1.60μM) and atranorin (IC50 = 4.41μM), while p-hydroxycinnamic acid was the least active (IC50 = 53.84μM). The EtOAc extract and its isolated compounds (specicoside and p-hydroxycinnamic acid) were non-cytotoxic (CC50 > 30μg/mL), whereas the n-hexane extract and two of its products, atranorin and 2β, 3β, 19α-trihydroxy-urs-12-en-28-oic acid showed cytotoxicity at high concentrations, with the last one being the most toxic (CC50 = 9.37μg/mL). These findings justify the use of K. africana stem bark as antimalaria by traditional healers of Western Cameroon, and could constitute a good basis for further studies towards development of new leads or natural drugs for malaria. Springer-Verlag, 2011 Zofou Denis Biotechnology Unit, University of Buea, P.O. Box63 Buea, South West Region, Cameroon aut Kengne Archile Laboratory of Natural Products Chemistry, University of Dschang, West Region, Cameroon aut Tene Mathieu Laboratory of Natural Products Chemistry, University of Dschang, West Region, Cameroon aut Ngemenya Moses Biotechnology Unit, University of Buea, P.O. Box63 Buea, South West Region, Cameroon aut Tane Pierre Laboratory of Natural Products Chemistry, University of Dschang, West Region, Cameroon aut Titanji Vincent Biotechnology Unit, University of Buea, P.O. Box63 Buea, South West Region, Cameroon aut Parasitology Research Springer-Verlag 108/6(2011-06-01), 1383-1390 0932-0113 108:6<1383 2011 108 436 https://doi.org/10.1007/s00436-011-2363-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2363-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zofou Denis Biotechnology Unit, University of Buea, P.O. Box63 Buea, South West Region, Cameroon aut NATIONALLICENCE 700 1- Kengne Archile Laboratory of Natural Products Chemistry, University of Dschang, West Region, Cameroon aut NATIONALLICENCE 700 1- Tene Mathieu Laboratory of Natural Products Chemistry, University of Dschang, West Region, Cameroon aut NATIONALLICENCE 700 1- Ngemenya Moses Biotechnology Unit, University of Buea, P.O. Box63 Buea, South West Region, Cameroon aut NATIONALLICENCE 700 1- Tane Pierre Laboratory of Natural Products Chemistry, University of Dschang, West Region, Cameroon aut NATIONALLICENCE 700 1- Titanji Vincent Biotechnology Unit, University of Buea, P.O. Box63 Buea, South West Region, Cameroon aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/6(2011-06-01), 1383-1390 0932-0113 108:6<1383 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer