caa a22 4500 44534217X CHVBK 20180317142816.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00436-010-2207-1 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2207-1 Detection of malaria infection in blood transfusion: a comparative study among real-time PCR, rapid diagnostic test and microscopy [Elektronische Daten] Sensitivity of Malaria detection methods in blood transfusion [Gholamreza Hassanpour, Mehdi Mohebali, Ahmad Raeisi, Hassan Abolghasemi, Hojjat Zeraati, Mohsen Alipour, Ebrahim Azizi, Hossein Keshavarz] The transmission of malaria by blood transfusion was one of the first transfusion-transmitted infections recorded in the world. Transfusion-transmitted malaria may lead to serious problems because infection with Plasmodium falciparum may cause rapidly fatal death. This study aimed to compare real-time polymerase chain reaction (real-time PCR) with rapid diagnostic test (RDT) and light microscopy for the detection of Plasmodium spp. in blood transfusion, both in endemic and non-endemic areas of malaria disease in Iran. Two sets of 50 blood samples were randomly collected. One set was taken from blood samples donated in blood bank of Bandar Abbas, a city located in a malarious-endemic area, and the other set from Tehran, a non-endemic one. Light microscopic examination on both thin and thick smears, RDTs, and real-time PCR were performed on the blood samples and the results were compared. Thin and thick light microscopic examinations of all samples as well as RDT results were negative for Plasmodium spp. Two blood samples from endemic area were positive only with real-time PCR. It seems that real-time PCR as a highly sensitive method can be helpful for the confirmation of malaria infection in different units of blood transfusion organization especially in malaria-endemic areas where the majority of donors may be potentially infected with malaria parasites. Springer-Verlag, 2011 Hassanpour Gholamreza Medical Parasitology and Mycology Department, School of Public Health, Tehran University of Medical Sciences, 14155-6446, Tehran, Iran aut Mohebali Mehdi Medical Parasitology and Mycology Department, School of Public Health, Tehran University of Medical Sciences, 14155-6446, Tehran, Iran aut Raeisi Ahmad Medical Entomology and Vector Control Department School of Public Health, Tehran University of Medical Sciences, Tehran, Iran aut Abolghasemi Hassan Iranian Blood Transfusion Organization, Tehran, Iran aut Zeraati Hojjat Epidemiology and Biostatistics Department School of Public Health, Tehran University of Medical Sciences, Tehran, Iran aut Alipour Mohsen Molecular Research Lab, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran aut Azizi Ebrahim Molecular Research Lab, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran aut Keshavarz Hossein Medical Parasitology and Mycology Department, School of Public Health, Tehran University of Medical Sciences, 14155-6446, Tehran, Iran aut Parasitology Research Springer-Verlag 108/6(2011-06-01), 1519-1523 0932-0113 108:6<1519 2011 108 436 https://doi.org/10.1007/s00436-010-2207-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2207-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hassanpour Gholamreza Medical Parasitology and Mycology Department, School of Public Health, Tehran University of Medical Sciences, 14155-6446, Tehran, Iran aut NATIONALLICENCE 700 1- Mohebali Mehdi Medical Parasitology and Mycology Department, School of Public Health, Tehran University of Medical Sciences, 14155-6446, Tehran, Iran aut NATIONALLICENCE 700 1- Raeisi Ahmad Medical Entomology and Vector Control Department School of Public Health, Tehran University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Abolghasemi Hassan Iranian Blood Transfusion Organization, Tehran, Iran aut NATIONALLICENCE 700 1- Zeraati Hojjat Epidemiology and Biostatistics Department School of Public Health, Tehran University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Alipour Mohsen Molecular Research Lab, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Azizi Ebrahim Molecular Research Lab, Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Keshavarz Hossein Medical Parasitology and Mycology Department, School of Public Health, Tehran University of Medical Sciences, 14155-6446, Tehran, Iran aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/6(2011-06-01), 1519-1523 0932-0113 108:6<1519 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer