caa a22 4500 445342188 CHVBK 20180317142817.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00436-010-2201-7 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2201-7 Characterization and localization of saposin-like protein-2 (SAP-2) in Fasciola gigantica [Elektronische Daten] [Pornanan Kueakhai, Krai Meemon, Narin Changklungmoa, Kulathida Chaithirayanon, Suda Riengrojpitak, Prasert Sobhon] Fasciola gigantica saposin-like protein-2 (FgSAP-2) belongs to a family of lipid-interacting proteins that are involved in the cytolysis of target cells. In this study, we have cloned and expressed FgSAP-2 and produced the antibody against this recombinant protein. Rabbit antiserum against rFgSAP-2 reacted with a similar native protein in the whole body extracts of the 4-week-old juvenile and adult stage, as well as a protein in their excretion-secretion, but not in the tegument. In situ hybridization and immunofluorescence detection revealed the presence of SAP-2 mRNA transcripts and proteins in the cecal epithelial cells of 4-week-old juvenile and adult parasites, but not in the metacercariae and newly excysted juveniles. Moreover, SAP-2 is present only in the cecal epithelial cells lining the distal part of the digestive tract, but not in the tegumental-type epithelium lining the proximal part of the digestive tract. The rFgSAP-2 reacted with antisera from rabbits infected with F. gigantica metacercariae collected at 5weeks, but not at 2weeks after infection. Anti-rFgSAP-2 did not exhibit any cross-reactivity with the other parasites' antigens, including Opisthorchis viverrini, Eurytrema pancreaticum, Cotylophoron cotylophorum, Fischoederius cobboldi, Gigantocotyle explanatum, Paramphistomum cervi, Setaria labiato-papillosa, and Haemonchus placei. This finding indicated that SAP-2 is a unique protein that is expressed only in late juvenile and adult F. gigantica, and it could be considered for immunodiagnostic and as a vaccine candidate for fasciolosis. Springer-Verlag, 2010 Kueakhai Pornanan Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut Meemon Krai Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut Changklungmoa Narin Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut Chaithirayanon Kulathida Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut Riengrojpitak Suda Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut Sobhon Prasert Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut Parasitology Research Springer-Verlag 108/6(2011-06-01), 1493-1500 0932-0113 108:6<1493 2011 108 436 https://doi.org/10.1007/s00436-010-2201-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2201-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kueakhai Pornanan Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Meemon Krai Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Changklungmoa Narin Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Chaithirayanon Kulathida Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Riengrojpitak Suda Department of Pathobiology, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Sobhon Prasert Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Rd., 10400, Bangkok, Thailand aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/6(2011-06-01), 1493-1500 0932-0113 108:6<1493 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer