caa a22 4500 44534220X CHVBK 20180317142817.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00436-010-2246-7 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2246-7 The typing of Trypanosoma evansi isolates using mobile genetic element (MGE) PCR [Elektronische Daten] [Z. Njiru, P. Gitonga, K. Ndungu] The mobile genetic element PCR (MGE-PCR) is a simple and sensitive technique that can be used to detect genetic variability in Trypanosoma brucei ssp. To investigate the reliability of MGE-PCR in genotyping Trypanosoma evansi, stocks that were isolated directly from camels and after their respective passage in mice were analyzed. Construction of a dendrogram using the MGE-PCR banding profiles revealed a clear distinction between T. evansi and T. brucei, as well as discriminating the T. evansi strains (T. evansi with minicircle types B and A). A minor host-dependent clustering shows a genetic difference of <15%. Changes in the banding profiles were observed after serial passage of T. evansi type B in mice, while those of T. evansi type A were identical. It is apparent that significant random insertion mobile element positional variation occurs when T. evansi isolates are introduced into a new host, a factor that needs to be considered when MGE-PCR is used to determine genetic variation in T. evansi isolates that have different host origins. Springer-Verlag, 2011 Njiru Z. School of Veterinary Sciences, University of Queensland, Inner Ring Road, 4343, Gatton, Australia aut Gitonga P. Trypanosomiasis Research Centre, Kenya Agricultural Research Institute, P.O. Box 362-00902, Kikuyu, Kenya aut Ndungu K. Trypanosomiasis Research Centre, Kenya Agricultural Research Institute, P.O. Box 362-00902, Kikuyu, Kenya aut Parasitology Research Springer-Verlag 108/6(2011-06-01), 1583-1587 0932-0113 108:6<1583 2011 108 436 https://doi.org/10.1007/s00436-010-2246-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2246-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Njiru Z. School of Veterinary Sciences, University of Queensland, Inner Ring Road, 4343, Gatton, Australia aut NATIONALLICENCE 700 1- Gitonga P. Trypanosomiasis Research Centre, Kenya Agricultural Research Institute, P.O. Box 362-00902, Kikuyu, Kenya aut NATIONALLICENCE 700 1- Ndungu K. Trypanosomiasis Research Centre, Kenya Agricultural Research Institute, P.O. Box 362-00902, Kikuyu, Kenya aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/6(2011-06-01), 1583-1587 0932-0113 108:6<1583 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer