caa a22 4500 445342242 CHVBK 20180317142817.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s00436-010-2209-z doi (NATIONALLICENCE)springer-10.1007/s00436-010-2209-z A fluorescence-based polymerase chain reaction-linked single-strand conformation polymorphism (F-PCR-SSCP) assay for the identification of Fasciola spp [Elektronische Daten] [Samer Alasaad, Ramón Soriguer, Marawan Abu-Madi, Ahmed El Behairy, Pablo Baños, Ana Píriz, Joerns Fickel, Xing-Quan Zhu] The present study aimed to establish a fluorescence-based polymerase chain reaction-linked single-strand conformation polymorphism (F-PCR-SSCP) assay for the identification of Fasciola spp. Based on the sequences of the second internal transcribed spacer (ITS-2) of the nuclear ribosomal DNA, we designed a set of genus-specific primers for the amplification of Fasciola ITS-2, with an estimated size of 140bp. These primers were labelled by fluorescence dyes, and the PCR products were analyzed by capillary electrophoresis under non-denaturing conditions (F-PCR-SSCP). Capillary electrophoresis analysis of the fluorescence-labelled DNA fragments displayed three different peak profiles that allowed the accurate identification of Fasciola species: one single peak specific for either Fasciola hepatica or Fasciola gigantica and a doublet peak corresponding to the "intermediate” Fasciola. Validation of our novel method was performed using Fasciola specimens from different host animals from China, Spain, Nigeria, and Egypt. This F-PCR-SSCP assay provides a rapid, simple, and robust tool for the identification and differentiation between Fasciola spp. Springer-Verlag, 2010 Alasaad Samer Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Américo Vespucio s/n, 41092, Seville, Spain aut Soriguer Ramón Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Américo Vespucio s/n, 41092, Seville, Spain aut Abu-Madi Marawan Department of Health Sciences, College of Arts and Sciences, Qatar University, Doha, Qatar aut El Behairy Ahmed Parasitology Department, Faculty of Veterinary Medicine, Cairo University, Cairo, Arab Republic of Egypt aut Baños Pablo Departamento de Patología Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27071, Lugo, Spain aut Píriz Ana Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Américo Vespucio s/n, 41092, Seville, Spain aut Fickel Joerns Research Group Evolutionary Genetics, Leibniz-Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315, Berlin, Germany aut Zhu Xing-Quan State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, 730046, Lanzhou, Gansu Province, Peoples Republic of China aut Parasitology Research Springer-Verlag 108/6(2011-06-01), 1513-1517 0932-0113 108:6<1513 2011 108 436 https://doi.org/10.1007/s00436-010-2209-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2209-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alasaad Samer Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Américo Vespucio s/n, 41092, Seville, Spain aut NATIONALLICENCE 700 1- Soriguer Ramón Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Américo Vespucio s/n, 41092, Seville, Spain aut NATIONALLICENCE 700 1- Abu-Madi Marawan Department of Health Sciences, College of Arts and Sciences, Qatar University, Doha, Qatar aut NATIONALLICENCE 700 1- El Behairy Ahmed Parasitology Department, Faculty of Veterinary Medicine, Cairo University, Cairo, Arab Republic of Egypt aut NATIONALLICENCE 700 1- Baños Pablo Departamento de Patología Animal, Parasitología y Enfermedades Parasitarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27071, Lugo, Spain aut NATIONALLICENCE 700 1- Píriz Ana Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (CSIC), Avda. Américo Vespucio s/n, 41092, Seville, Spain aut NATIONALLICENCE 700 1- Fickel Joerns Research Group Evolutionary Genetics, Leibniz-Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315, Berlin, Germany aut NATIONALLICENCE 700 1- Zhu Xing-Quan State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, 730046, Lanzhou, Gansu Province, Peoples Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/6(2011-06-01), 1513-1517 0932-0113 108:6<1513 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer