caa a22 4500 44534234X CHVBK 20180317142817.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00436-010-2099-0 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2099-0 RNAi silencing of calcium-regulated heat-stable protein of 24kDa in Schistosoma japonicum affects parasite growth [Elektronische Daten] [Xiang Zou, Ya-mei Jin, Ping-ping Liu, Qi-jin Wu, Jin-ming Liu, Jiao-jiao Lin] The calcium-regulated heat-stable protein of 24kDa (CRHSP-24) is a major calcineurin phosphoprotein that functions in multiple signal transduction pathways in cell metabolism. Schistosomes are multicellular parasites that infect 200million people worldwide, even though treatment has been available for two decades. To determine the function of schistosome CRHSP-24 (SjCRHSP-24), we successfully knocked down SjCRHSP-24 in Schistosoma japonicum by RNA interference (RNAi). By establishing controls for measuring off-target RNAi effects, we found that different double-stranded (dsRNA) sequences had different levels of effectiveness. While all tested dsRNAs reduced CRHSP-24 transcript levels, the S2 dsRNA consistently reduced CRHSP-24 expression to >95% of the control. Knockdown of the SjCRHSP-24 gene significantly affected the morphology and vitality of S. japonicum. Springer-Verlag, 2010 Zou Xiang Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut Jin Ya-mei Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut Liu Ping-ping Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut Wu Qi-jin Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut Liu Jin-ming Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut Lin Jiao-jiao Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut Parasitology Research Springer-Verlag 108/3(2011-03-01), 567-572 0932-0113 108:3<567 2011 108 436 https://doi.org/10.1007/s00436-010-2099-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2099-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zou Xiang Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Jin Ya-mei Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Ping-ping Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wu Qi-jin Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Jin-ming Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Lin Jiao-jiao Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences; Key Laboratory of Animal Parasitology, Ministry of Agriculture of China, No. 518, Ziyue Road, 200241, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/3(2011-03-01), 567-572 0932-0113 108:3<567 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer