caa a22 4500 445342382 CHVBK 20180317142817.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00436-010-2096-3 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2096-3 Morphological alterations and growth inhibition of Leishmania (L.)amazonensis promastigotes exposed to zidovudine (AZT) [Elektronische Daten] [Carolina Araújo, Aline Araújo, Camilla Batista, Milton Oliveira, Valeria Oliveira, Ruy Lino Junior, Marina Vinaud, Jose Bezerra] Leishmania parasites cause a worldwide public health disease and its treatment is still based on pentavalent antimonials which present financial and toxicologic limitations. Some nucleosidic derivatives have demonstrated anti-leishmanial properties and this study aims to evaluate the in vitro morphologic alterations and growth inhibition of Leishmania (L.) amazonensis promastigotes exposed to zidovudine at several concentrations. The citotoxicity of zidovudine (AZT) to macrophages was determined by an MTT assay. After which the promastigotes were exposed to concentrations of AZT, ranging from 1 to 50μM. The evaluation of survival and morphometry alterations were performed in two distinct phases of in vitro growth, on the third and sixth days, representing the logarithmic and stationary phases, respectively. Slides with the promastigotes were photographed and analyzed using Image J. A significant reduction of parasite number in the logarithmic phase of in vitro growth was observed when the parasites were submitted to 20, 30, 40, and 50μM of AZT. Morphometric alterations were observed such as an increase in width of the body, cytoplasmic granulations and vacuolizations. These data indicate the toxicity of AZT which prevents the parasite's multiplication, indicating a promising use of AZT as an anti-leishmania drug. Springer-Verlag, 2010 Araújo Carolina Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut Araújo Aline Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut Batista Camilla Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut Oliveira Milton Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut Oliveira Valeria Bioconversion Laboratory, Pharmacy Faculty, Federal University of Goiás, Av. Universitária com 1ª Avenida s/n, Setor Universitário, Goiânia, GO, Brazil aut Lino Junior Ruy Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut Vinaud Marina Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut Bezerra Jose Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut Parasitology Research Springer-Verlag 108/3(2011-03-01), 547-551 0932-0113 108:3<547 2011 108 436 https://doi.org/10.1007/s00436-010-2096-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2096-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Araújo Carolina Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut NATIONALLICENCE 700 1- Araújo Aline Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut NATIONALLICENCE 700 1- Batista Camilla Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut NATIONALLICENCE 700 1- Oliveira Milton Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut NATIONALLICENCE 700 1- Oliveira Valeria Bioconversion Laboratory, Pharmacy Faculty, Federal University of Goiás, Av. Universitária com 1ª Avenida s/n, Setor Universitário, Goiânia, GO, Brazil aut NATIONALLICENCE 700 1- Lino Junior Ruy Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut NATIONALLICENCE 700 1- Vinaud Marina Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut NATIONALLICENCE 700 1- Bezerra Jose Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, rua 235, s/n, Setor Universitário, CEP: 74650-050, Goiania, GO, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/3(2011-03-01), 547-551 0932-0113 108:3<547 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer