caa a22 4500 445342447 CHVBK 20180317142817.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00436-010-2102-9 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2102-9 Protein change of intestinal epithelial cells induced in vitro by Trichinella spiralis infective larvae [Elektronische Daten] [Shu Wang, Zhong Wang, Jing Cui] The aim of this study was to observe the protein changes of intestinal epithelial cells induced in vitro by Trichinella spiralis infective larvae and their excretory-secretory (ES) or surface antigens and identity the proteins related with invasion. HCT-8 cells were incubated for 2h in the culture medium contained ES or surface antigens of infective larvae, and observed by Immunofluorescent test (IFT). The infective larvae were inoculated into culture of HCT-8 cells to incubate for 18h, and the lysates of HCT-8 cells were analyzed by SDS-PAGE and Western blot. IFA showed that normal HCT-8 cells had positively reactions with sera of the infected mice and mice immunized with ES or surface antigens. However, after incubating with ES or surface antigens, HCT-8 cells had stronger positively reaction with the above sera. On Western blot, after cultured with infective larvae, additional seven protein bands (66, 61, 57, 45, 34, 21, and 17kDa) of HCT-8 cells were recognized by sera of the infected or immunized mice, but three protein bands (48, 43, and 23kDa) of HCT-8 cells were not recognized by the above sera, compared with normal HCT-8 cells. Our results showed that after cultured with infective larvae the protein components of HCT-8 cell changed, suggesting that additional seven proteins recognized by sera of the infected or immunized mice may be related with invasion of intestinal epithelial cells by infective larvae, these proteins might mediate or facilitate entry into the cells, while the three proteins not recognized by the above sera may be the specific mediators released from the cells which permit invasion. Springer-Verlag, 2010 Wang Shu Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut Wang Zhong Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut Cui Jing Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut Parasitology Research Springer-Verlag 108/3(2011-03-01), 593-599 0932-0113 108:3<593 2011 108 436 https://doi.org/10.1007/s00436-010-2102-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2102-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Shu Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zhong Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cui Jing Department of Parasitology, Medical College, Zhengzhou University, 450052, Zhengzhou, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/3(2011-03-01), 593-599 0932-0113 108:3<593 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer