caa a22 4500 445342455 CHVBK 20180317142817.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00436-010-2104-7 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2104-7 Eimeria maxima phosphatidylinositol 4-phosphate 5-kinase: locus sequencing, characterization, and cross-phylum comparison [Elektronische Daten] [Mei-Yen Goh, Mei-Zhen Pan, Damer Blake, Kiew-Lian Wan, Beng-Kah Song] Phosphatidylinositol 4-phosphate 5-kinase (PIP5K) may play an important role in host-cell invasion by the Eimeria species, protozoan parasites which can cause severe intestinal disease in livestock. Here, we report the structural organization of the PIP5K gene in Eimeria maxima (Weybridge strain). Two E. maxima BAC clones carrying the E. maxima PIP5K (EmPIP5K) coding sequences were selected for shotgun sequencing, yielding a 9.1-kb genomic segment. The EmPIP5K coding region was initially identified using in silico gene-prediction approaches and subsequently confirmed by mapping rapid amplification of cDNA ends and RT-PCR-generated cDNA sequence to its genomic segment. The putative EmPIP5K gene was located at position 710-8036 nt on the complimentary strand and comprised of 23 exons. Alignment of the 1147 amino acid sequence with previously annotated PIP5K proteins from other Apicomplexa species detected three conserved motifs encompassing the kinase core domain, which has been shown by previous protein deletion studies to be necessary for PIP5K protein function. Phylogenetic analysis provided further evidence that the putative EmPIP5K protein is orthologous to that of other Apicomplexa. Subsequent comparative gene structure characterization revealed events of intron loss/gain throughout the evolution of the apicomplexan PIP5K gene. Further scrutiny of the genomic structure revealed a possible trend towards "intron gain” between two of the motif regions. Our findings offer preliminary insights into the structural variations that have occurred during the evolution of the PIP5K locus and may aid in understanding the functional role of this gene in the cellular biology of apicomplexan parasites. Springer-Verlag, 2010 Goh Mei-Yen School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor DE, Malaysia aut Pan Mei-Zhen School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor DE, Malaysia aut Blake Damer Parasitology, Institute for Animal Health, Compton, RG20 7NN, Berkshire, UK aut Wan Kiew-Lian Malaysia Genome Institute, UKM-MTDC Technology Centre, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE, Malaysia aut Song Beng-Kah School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor DE, Malaysia aut Parasitology Research Springer-Verlag 108/3(2011-03-01), 611-620 0932-0113 108:3<611 2011 108 436 https://doi.org/10.1007/s00436-010-2104-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2104-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Goh Mei-Yen School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor DE, Malaysia aut NATIONALLICENCE 700 1- Pan Mei-Zhen School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor DE, Malaysia aut NATIONALLICENCE 700 1- Blake Damer Parasitology, Institute for Animal Health, Compton, RG20 7NN, Berkshire, UK aut NATIONALLICENCE 700 1- Wan Kiew-Lian Malaysia Genome Institute, UKM-MTDC Technology Centre, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor DE, Malaysia aut NATIONALLICENCE 700 1- Song Beng-Kah School of Science, Monash University Sunway Campus, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor DE, Malaysia aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/3(2011-03-01), 611-620 0932-0113 108:3<611 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer