caa a22 4500 445342501 CHVBK 20180317142818.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00436-010-2094-5 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2094-5 Elimination of Angiostrongylus costaricensis larvae in feces from experimentally infected Swiss mice: circadian rhythm and correlation with survival [Elektronische Daten] [Graciele de Azevedo, Rubens Rodriguez, Sérgio Porto, Carlos Graeff-Teixeira, Fernando Fornari] Angiostrongylus costaricensis is a nematode which harbors mesentery arteries of rodents. In these animals, a circadian rhythm of elimination of first-stage larvae (L1) and a relation between the amount of L1 in feces and survival are unknown. We assessed fecal elimination of A. costaricensis L1 from experimentally infected Swiss mice and tried to correlate L1 elimination with survival. Thirteen Swiss mice were infected by gavage with ten A. costaricensis L3 larvae obtained from Phyllocaulis slugs. Feces were weighed at 7a.m. and 7p.m. starting from the 24th day post-infection until animal death. Feces sediment was examined in microscope for L1 counting. The mice were dead after a period ranging 19-61days post-infection. Compared to diurnal samples, both feces' weight (2.3 ± 0.7 vs. 1.8 ± 0.5g; P < 0.0001) and L1 total count [median 1,950 vs. 1,250; P = 0.015] were higher in feces eliminated at night. No difference was observed between diurnal and nocturnal elimination when counting L1 by gram of feces (725 vs. 650L1/g; P = 0.821). A significant correlation was observed between survival and total number of L1 in feces (r = 0.84; P = 0.0007). This study suggests that mice experimentally infected with A. costaricensis eliminate more L1 at night due to higher fecal volume at this period. The correlation between number of L1 in feces and survival suggests a phenomenon of tolerance to A. costaricensis infection in mice with longer survival. Springer-Verlag, 2010 de Azevedo Graciele Instituto de Ciências Biológicas (ICB), Universidade de Passo Fundo (UPF), Passo Fundo, RS, Brazil aut Rodriguez Rubens Programa de Pós-Graduação: Ciências em Gastroenterologia, Faculdade de Medicina, UFRGS, Porto Alegre, RS, Brazil aut Porto Sérgio Instituto de Ciências Biológicas (ICB), Universidade de Passo Fundo (UPF), Passo Fundo, RS, Brazil aut Graeff-Teixeira Carlos Laboratório de Biologia Parasitária, Faculdade de Biociências, PUCRS, Porto Alegre, RS, Brazil aut Fornari Fernando Programa de Pós-Graduação: Ciências em Gastroenterologia, Faculdade de Medicina, UFRGS, Porto Alegre, RS, Brazil aut Parasitology Research Springer-Verlag 108/3(2011-03-01), 537-540 0932-0113 108:3<537 2011 108 436 https://doi.org/10.1007/s00436-010-2094-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2094-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- de Azevedo Graciele Instituto de Ciências Biológicas (ICB), Universidade de Passo Fundo (UPF), Passo Fundo, RS, Brazil aut NATIONALLICENCE 700 1- Rodriguez Rubens Programa de Pós-Graduação: Ciências em Gastroenterologia, Faculdade de Medicina, UFRGS, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Porto Sérgio Instituto de Ciências Biológicas (ICB), Universidade de Passo Fundo (UPF), Passo Fundo, RS, Brazil aut NATIONALLICENCE 700 1- Graeff-Teixeira Carlos Laboratório de Biologia Parasitária, Faculdade de Biociências, PUCRS, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Fornari Fernando Programa de Pós-Graduação: Ciências em Gastroenterologia, Faculdade de Medicina, UFRGS, Porto Alegre, RS, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/3(2011-03-01), 537-540 0932-0113 108:3<537 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer