caa a22 4500 445342528 CHVBK 20180317142818.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00436-010-2180-8 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2180-8 Genetic characterization of Echinostoma revolutum and Echinoparyphium recurvatum (Trematoda: Echinostomatidae) in Thailand and phylogenetic relationships with other isolates inferred by ITS1 sequence [Elektronische Daten] [Weerachai Saijuntha, Chairat Tantrawatpan, Paiboon Sithithaworn, Ross Andrews, Trevor Petney] Echinostomatidae are common, widely distributed intestinal parasites causing significant disease in both animals and humans worldwide. In spite of their importance, the taxonomy of these echinostomes is still controversial. The taxonomic status of two species, Echinostoma revolutum and Echinoparyphium recurvatum, which commonly infect poultry and other birds, as well as human, is problematical. Previous phylogenetic analyses of Southeast Asian strains indicate that these species cluster as sister taxa. In the present study, the first internal transcribed spacer (ITS1) sequence was used for genetic characterization and to examine the phylogenetic relationships between an isolate from Thailand with other isolates available from GenBank database. Interspecies differences in ITS1 sequence between E. revolutum and E. recurvatum were detected at 6 (3%) of the 203 alignment positions. Of these, nucleotide deletion at positions 25, 26, and 27, pyrimidine transition at 50, 189, and pyrimidine transversion at 118 were observed. Phylogenetic analysis revealed that E. recurvatum from Thailand clustered as a sister taxa with E. revolutum and not with other members of the genus Echinoparyphium. Interestingly, this result confirms a previous report based on allozyme electrophoresis and mitochondrial DNA that E. revolutum and E. recurvatum in Southeast Asia are sister species. Hence, the taxonomic status of E. recurvatum in Thailand, as well as in Southeast Asian countries needs to be confirmed and revised using more comprehensive analyses based on morphology and other molecular techniques. Springer-Verlag, 2010 Saijuntha Weerachai Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, 44150, Maha Sarakham, Thailand aut Tantrawatpan Chairat Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, 12121, Pathumthani, Thailand aut Sithithaworn Paiboon Department of Parasitology, Liver Fluke and Cholangiocarcinoma Research Centre (LFCRC), Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Andrews Ross Department of Parasitology, Liver Fluke and Cholangiocarcinoma Research Centre (LFCRC), Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut Petney Trevor Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Kornblumen Strasse 13, Karlsruhe, Germany aut Parasitology Research Springer-Verlag 108/3(2011-03-01), 751-755 0932-0113 108:3<751 2011 108 436 https://doi.org/10.1007/s00436-010-2180-8 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2180-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saijuntha Weerachai Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, 44150, Maha Sarakham, Thailand aut NATIONALLICENCE 700 1- Tantrawatpan Chairat Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, 12121, Pathumthani, Thailand aut NATIONALLICENCE 700 1- Sithithaworn Paiboon Department of Parasitology, Liver Fluke and Cholangiocarcinoma Research Centre (LFCRC), Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Andrews Ross Department of Parasitology, Liver Fluke and Cholangiocarcinoma Research Centre (LFCRC), Faculty of Medicine, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Petney Trevor Department of Ecology and Parasitology, Karlsruhe Institute of Technology, Kornblumen Strasse 13, Karlsruhe, Germany aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/3(2011-03-01), 751-755 0932-0113 108:3<751 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer