caa a22 4500 445342536 CHVBK 20180317142818.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00436-010-2114-5 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2114-5 Bacteriocin-like substance from Bacillus amyloliquefaciens shows remarkable inhibition of Acanthamoeba polyphaga [Elektronische Daten] [Lisianne Benitez, Karin Caumo, Adriano Brandelli, Marilise Rott] The effectiveness of a bacteriocin-like substance (BLS) produced by Bacillus amyloliquefaciens was tested against Acanthamoeba polyphaga strains, and its cytotoxic potential on Vero cells was investigated. Amebicidal activity of the purified BLS was tested by plate bioassays with concentrations ranging from 12.5 to 6,400AUmL−1. Damage to A. pholyphaga cells was monitored using an inverted microscope and counted in a Fuchs-Rosenthal chamber after 24, 48, and 72h. According to the results obtained, the BLS showed remarkable amebicidal and amebostatic effect on A. polyphaga and showed no cytotoxicity on the Vero cells. These results may have great relevance in the development of new acanthamoebicidal compounds. Springer-Verlag, 2010 Benitez Lisianne Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Caumo Karin Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Brandelli Adriano Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, ICTA, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut Rott Marilise Departamento de Microbiologia, Setor de Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre, Brazil aut Parasitology Research Springer-Verlag 108/3(2011-03-01), 687-691 0932-0113 108:3<687 2011 108 436 https://doi.org/10.1007/s00436-010-2114-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2114-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Benitez Lisianne Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Caumo Karin Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Brandelli Adriano Laboratório de Bioquímica e Microbiologia Aplicada, Departamento de Ciência de Alimentos, ICTA, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil aut NATIONALLICENCE 700 1- Rott Marilise Departamento de Microbiologia, Setor de Parasitologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, 90050-170, Porto Alegre, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/3(2011-03-01), 687-691 0932-0113 108:3<687 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer