caa a22 4500 445342617 CHVBK 20180317142818.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00436-010-2108-3 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2108-3 Moczoń Tadeusz W. Stefański Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warszawa, Poland aut A cysteine proteinase in the penetration glands of the cercariae of Cotylurus cornutus (Trematoda, Strigeidae) [Elektronische Daten] [Tadeusz Moczoń] A cysteine proteinase from the penetration glands of Cotylurus cornutus cercariae was examined with histochemical and biochemical methods. The enzyme hydrolyzed gelatin, azocoll, azocasein, azoalbumin, N-blocked-l-arginine-4-methoxy-2-naphthylamide, and N-blocked-p-nitroanilide, but did not degrade elastin. The metal ion complexane ethylenediamine tetraacetate and the thiol-reducing compound dithioerythritol enhanced the proteinase activity, whereas the thiol-blocking compounds p-hydroxymercuribenzoate and N-ethylmaleimide (NEM) inhibited it. The enzyme was also sensitive to leupeptin but insensitive to soybean trypsin inhibitor. An electrophoretic separation of extract proteins from the cercariae under acidic, non-denaturing conditions and in the presence of 0.1% gelatin in a polyacrylamide gel revealed the presence of two distinct and three weak transparent bands in the gel resulting from a gelatinolytic activity at pH6.8. The distinct bands apparently resulted from the activity of the glandular enzyme and lysosomal cathepsin B, whereas the weak ones presumably indicated these enzymes partially degraded in the course of the preparative procedure. No gelatinolysis occurred following treatment of an extract sample with 0.1mM NEM. The Author(s), 2010 Parasitology Research Springer-Verlag 108/3(2011-03-01), 639-643 0932-0113 108:3<639 2011 108 436 https://doi.org/10.1007/s00436-010-2108-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2108-3 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Moczoń Tadeusz W. Stefański Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818, Warszawa, Poland aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/3(2011-03-01), 639-643 0932-0113 108:3<639 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer