caa a22 4500 445342625 CHVBK 20180317142818.0 cr unu---uuuuu 170323e20110301xx s 000 0 eng 10.1007/s00436-010-2109-2 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2109-2 Proteomic survey of the cestode Mesocestoides corti during the first 24 hours of strobilar development [Elektronische Daten] [Alice Laschuk, Karina Monteiro, Newton Vidal, Paulo Pinto, Rosario Duran, Carlos Cerveñanski, Arnaldo Zaha, Henrique Ferreira] Despite the fact that cestodes represent major etiological agents of both human and domestic animal diseases, little is known about the molecular aspects of cestode development. In this work, Mesocestoides corti, a model cestode species, was studied from the early development of its larval form (tetrathyridium) into adult worms (strobilation) using different proteomic approaches. The protein profiles of M. corti tetrathyridia induced or not induced to undergo strobilation were compared. Proteomic mapping by two-dimensional gel electrophoresis showed the resolution of 248 and 154 spots from tetrathyridia that were subjected or not subjected to strobilation induction, respectively, allowing for the detection of at least nine spots exclusive to each group. Spot analysis by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) or MALDI-TOF MS/MS identified four reference proteins (six spots). LC-MS/MS analyses of protein extracts identified 66 proteins, eight of which were found exclusively in non-induced tetrathyridia, while 13 were found exclusively in strobilation-induced tetrathyridia. Among the proteins exclusively identified in strobilation-induced worms, there was a predominance of proteins with functions relating to chaperone activity and protein synthesis and turnover. Quantitative differential expression analysis between M. corti tetrathyridia prior to and after strobilation induction revealed six proteins upregulated in strobilation-induced worms; these proteins were involved in metabolic pathways, cell proliferation, and cytoskeletal rearrangement. Overall, despite the absence of a sequenced M. corti genome, using sequences from other platyhelminthes, we were able to establish comprehensive protein profiles for tetrathyridia prior to and after strobilation induction and identify several proteins potentially involved in the early events leading to strobilation. Springer-Verlag, 2010 Laschuk Alice Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Bloco IV, prédio 43-421, Sala 206, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut Monteiro Karina Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Bloco IV, prédio 43-421, Sala 206, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut Vidal Newton Departamento de Genética, Programa de Pós-Graduação em Genética e Biologia Molecular, UFRGS, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut Pinto Paulo Laboratório de Genômica Estrutural e Funcional, CBiot, UFRGS, Av. Bento Gonçalves, 9500, Bloco IV, prédio 43-421, Sala 210, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut Duran Rosario Unit of Analytical Biochemistry and Proteomics, Institut Pasteur de Montevideo, Calle Mataojo 2020, 11400, Montevideo, Uruguay aut Cerveñanski Carlos Unit of Analytical Biochemistry and Proteomics, Institut Pasteur de Montevideo, Calle Mataojo 2020, 11400, Montevideo, Uruguay aut Zaha Arnaldo Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Bloco IV, prédio 43-421, Sala 206, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut Ferreira Henrique Laboratório de Biologia Molecular de Cestódeos and Laboratório de Genômica Estrutural e Funcional, CBiot, UFRGS, Av. Bento Gonçalves, 9500, Bloco IV, prédio 43-421, Salas 206/210, Cx. Postal 15005, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut Parasitology Research Springer-Verlag 108/3(2011-03-01), 645-656 0932-0113 108:3<645 2011 108 436 https://doi.org/10.1007/s00436-010-2109-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2109-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Laschuk Alice Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Bloco IV, prédio 43-421, Sala 206, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut NATIONALLICENCE 700 1- Monteiro Karina Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Bloco IV, prédio 43-421, Sala 206, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut NATIONALLICENCE 700 1- Vidal Newton Departamento de Genética, Programa de Pós-Graduação em Genética e Biologia Molecular, UFRGS, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut NATIONALLICENCE 700 1- Pinto Paulo Laboratório de Genômica Estrutural e Funcional, CBiot, UFRGS, Av. Bento Gonçalves, 9500, Bloco IV, prédio 43-421, Sala 210, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut NATIONALLICENCE 700 1- Duran Rosario Unit of Analytical Biochemistry and Proteomics, Institut Pasteur de Montevideo, Calle Mataojo 2020, 11400, Montevideo, Uruguay aut NATIONALLICENCE 700 1- Cerveñanski Carlos Unit of Analytical Biochemistry and Proteomics, Institut Pasteur de Montevideo, Calle Mataojo 2020, 11400, Montevideo, Uruguay aut NATIONALLICENCE 700 1- Zaha Arnaldo Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Bloco IV, prédio 43-421, Sala 206, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut NATIONALLICENCE 700 1- Ferreira Henrique Laboratório de Biologia Molecular de Cestódeos and Laboratório de Genômica Estrutural e Funcional, CBiot, UFRGS, Av. Bento Gonçalves, 9500, Bloco IV, prédio 43-421, Salas 206/210, Cx. Postal 15005, 91501-970, Porto Alegre, Rio Grande do Sul, Brazil aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/3(2011-03-01), 645-656 0932-0113 108:3<645 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer