caa a22 4500 44534265X CHVBK 20180317142818.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-010-2230-2 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2230-2 Antileishmanial efficacy of fluconazole and miltefosine in combination with an immunomodulator—picroliv [Elektronische Daten] [Nishi Shakya, Shraddha Sane, Suman Gupta] The chemotherapy of visceral leishmaniasis (VL) has several limitations including resistance and toxicity of the existing drugs. Downregulation of immune system further aggravates the problems. To combat this situation, leishmanicidal efficacy of already marketed standard antifungal drug, fluconazole under the approach of "therapeutic switching” in combination with standard antileishmanial drug, miltefosine, and a potent immunomodulator agent, picroliv, were evaluated in hamsters infected with Leishmania donovani. Animals treated with fluconazole (50mg/kg × 5days, oral (p.o.)) + miltefosine (5mg/kg × 5days, p.o.) showed enhancement in antileishmanial efficacy (77%), reactive nitrogen species, reactive oxygen species, hydrogen peroxide, and phagocytosis index as compared to those treated with individual drugs. Addition of picroliv to this combination further increased the antileishmanial efficacy from 77% to 88%. Upregulation of cell-mediated immunity was also observed in animals of this group which strengthens the immunomodulatory role of picroliv. These findings suggest a new option for antileishmanial chemotherapy at lower cost and toxicity. Springer-Verlag, 2011 Shakya Nishi Division of Parasitology, Central Drug Research Institute (CSIR), Chattar Manzil Palace, M.G. Road, 226001, Lucknow, Uttar Pradesh, India aut Sane Shraddha Division of Parasitology, Central Drug Research Institute (CSIR), Chattar Manzil Palace, M.G. Road, 226001, Lucknow, Uttar Pradesh, India aut Gupta Suman Division of Parasitology, Central Drug Research Institute (CSIR), Chattar Manzil Palace, M.G. Road, 226001, Lucknow, Uttar Pradesh, India aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 793-800 0932-0113 108:4<793 2011 108 436 https://doi.org/10.1007/s00436-010-2230-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2230-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shakya Nishi Division of Parasitology, Central Drug Research Institute (CSIR), Chattar Manzil Palace, M.G. Road, 226001, Lucknow, Uttar Pradesh, India aut NATIONALLICENCE 700 1- Sane Shraddha Division of Parasitology, Central Drug Research Institute (CSIR), Chattar Manzil Palace, M.G. Road, 226001, Lucknow, Uttar Pradesh, India aut NATIONALLICENCE 700 1- Gupta Suman Division of Parasitology, Central Drug Research Institute (CSIR), Chattar Manzil Palace, M.G. Road, 226001, Lucknow, Uttar Pradesh, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 793-800 0932-0113 108:4<793 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer