caa a22 4500 445342714 CHVBK 20180317142818.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-010-2121-6 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2121-6 Histopathological studies in two strains of semi-immune mice infected with Plasmodium berghei ANKA after chronic exposure [Elektronische Daten] [Gideon Helegbe, Tetsuo Yanagi, Masachika Senba, Nguyen Huy, Mohammed Shuaibu, Akiko Yamazaki, Mihoko Kikuchi, Michio Yasunami, Kenji Hirayama] To mimic a human malaria infection in the endemic condition, two strains of mice (Balb/c and CBA) were infected and treated several times to generate so-called semi-immune status. As previously reported, neither mice (Balb/c and CBA) strain showed cerebral malaria, even in the susceptible C57BL/6 (B6). The significant difference between the mice strains in our previous study was the rate of destruction of uninfected red blood cells (uRBCs) at infection. After the established repeated cycles of infection and treatment and the final challenge with 104 Plasmodium berghei ANKA until minimum Hb, Balb/c and CBA mice were sacrificed. The spleen, liver, brain, kidney, lung, heart, and muscle were removed, stained with hematoxylin-eosin and analyzed with light microscopy. Previous observation suggested that Balb/c destroyed uRBC at much higher rate than the other strains although the parasitemia was very low. Pathological investigation carried out in this study revealed that this destruction was mainly contributed by the uRBCs as no parasite sequestration was observed in any of the organs. However, malaria pigment deposition was observed in spleen and liver of all the semi-immune mice strains. This histopathological study in the severe malaria anemia model, which is difficult to conduct in humans, will be helpful in taking into account different responses to malaria infection when designing therapeutic interventions and vaccine studies. Springer-Verlag, 2010 Helegbe Gideon Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Yanagi Tetsuo Animal Research Centre for Tropical Infections, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Senba Masachika Department of Pathology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Huy Nguyen Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Shuaibu Mohammed Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Yamazaki Akiko Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Kikuchi Mihoko Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Yasunami Michio Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Hirayama Kenji Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 807-814 0932-0113 108:4<807 2011 108 436 https://doi.org/10.1007/s00436-010-2121-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2121-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Helegbe Gideon Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Yanagi Tetsuo Animal Research Centre for Tropical Infections, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Senba Masachika Department of Pathology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Huy Nguyen Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Shuaibu Mohammed Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Yamazaki Akiko Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Kikuchi Mihoko Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Yasunami Michio Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 700 1- Hirayama Kenji Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, 852-8523, Nagasaki, Japan aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 807-814 0932-0113 108:4<807 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer