caa a22 4500 445342749 CHVBK 20180317142818.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-010-2127-0 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2127-0 Valeriana wallichii root extracts and fractions with activity against Leishmania spp [Elektronische Daten] [Subhalakshmi Ghosh, Sukalyani Debnath, Sudipta Hazra, Andreas Hartung, Katja Thomale, Martina Schultheis, Petra Kapkova, Uta Schurigt, Heidrun Moll, Ulrike Holzgrabe, Banasri Hazra] Leishmanial diseases, posing a public health problem worldwide, are caused by Leishmania parasites with a dimorphic life cycle alternating between the promastigote and amastigote forms. Promastigotes transmitted by the vector are transformed into amastigotes residing in the host tissue macrophages. Presently, new antiparasitic agents are needed against Leishmania donovani and Leishmania major, the respective organisms causing visceral and cutaneous leishmaniasis, since the available treatments are unsatisfactory due to toxicity, high cost, and emerging drug resistance. Over the years, traditional medicinal flora throughout the world enriched the modern pharmacopeia. Hence, roots of ‘Indian Valerian' (Valeriana wallichii DC) were studied for its antileishmanial activity for the first time. The methanol and chloroform extracts showed activity against L. donovani promastigotes and both promastigotes and amastigotes of L. major. The most active fraction, F3, obtained from the chloroform extract, showed IC50 at ∼3-7μg/ml against both the promastigotes and 0.3μg/ml against L. major amastigotes. On investigation of the mechanism of cytotoxicity in L. donovani promastigotes, the ‘hall-mark' events of morphological degeneration, DNA fragmentation, externalization of phosphatidyl serine, and mitochondrial membrane depolarization indicated that F3 could induce apoptotic death in leishmanial cells. Therefore, the present study revealed a novel and unconventional property of V. wallichii root as a prospective source of effective antileishmanial agents. Springer-Verlag, 2010 Ghosh Subhalakshmi Department of Pharmaceutical Technology, Jadavpur University, 700032, Kolkata, India aut Debnath Sukalyani Department of Pharmaceutical Technology, Jadavpur University, 700032, Kolkata, India aut Hazra Sudipta Department of Pharmaceutical Technology, Jadavpur University, 700032, Kolkata, India aut Hartung Andreas Institute of Pharmacy and Food Chemistry, University of Würzburg, Am Hubland, 97074, Würzburg, Germany aut Thomale Katja Institute for Molecular Infection Biology, University of Würzburg, Josef-Schneider-Str. 2, 97080, Würzburg, Germany aut Schultheis Martina Institute for Molecular Infection Biology, University of Würzburg, Josef-Schneider-Str. 2, 97080, Würzburg, Germany aut Kapkova Petra Institute of Pharmacy and Food Chemistry, University of Würzburg, Am Hubland, 97074, Würzburg, Germany aut Schurigt Uta Institute for Molecular Infection Biology, University of Würzburg, Josef-Schneider-Str. 2, 97080, Würzburg, Germany aut Moll Heidrun Institute for Molecular Infection Biology, University of Würzburg, Josef-Schneider-Str. 2, 97080, Würzburg, Germany aut Holzgrabe Ulrike Institute of Pharmacy and Food Chemistry, University of Würzburg, Am Hubland, 97074, Würzburg, Germany aut Hazra Banasri Department of Pharmaceutical Technology, Jadavpur University, 700032, Kolkata, India aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 861-871 0932-0113 108:4<861 2011 108 436 https://doi.org/10.1007/s00436-010-2127-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2127-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ghosh Subhalakshmi Department of Pharmaceutical Technology, Jadavpur University, 700032, Kolkata, India aut NATIONALLICENCE 700 1- Debnath Sukalyani Department of Pharmaceutical Technology, Jadavpur University, 700032, Kolkata, India aut NATIONALLICENCE 700 1- Hazra Sudipta Department of Pharmaceutical Technology, Jadavpur University, 700032, Kolkata, India aut NATIONALLICENCE 700 1- Hartung Andreas Institute of Pharmacy and Food Chemistry, University of Würzburg, Am Hubland, 97074, Würzburg, Germany aut NATIONALLICENCE 700 1- Thomale Katja Institute for Molecular Infection Biology, University of Würzburg, Josef-Schneider-Str. 2, 97080, Würzburg, Germany aut NATIONALLICENCE 700 1- Schultheis Martina Institute for Molecular Infection Biology, University of Würzburg, Josef-Schneider-Str. 2, 97080, Würzburg, Germany aut NATIONALLICENCE 700 1- Kapkova Petra Institute of Pharmacy and Food Chemistry, University of Würzburg, Am Hubland, 97074, Würzburg, Germany aut NATIONALLICENCE 700 1- Schurigt Uta Institute for Molecular Infection Biology, University of Würzburg, Josef-Schneider-Str. 2, 97080, Würzburg, Germany aut NATIONALLICENCE 700 1- Moll Heidrun Institute for Molecular Infection Biology, University of Würzburg, Josef-Schneider-Str. 2, 97080, Würzburg, Germany aut NATIONALLICENCE 700 1- Holzgrabe Ulrike Institute of Pharmacy and Food Chemistry, University of Würzburg, Am Hubland, 97074, Würzburg, Germany aut NATIONALLICENCE 700 1- Hazra Banasri Department of Pharmaceutical Technology, Jadavpur University, 700032, Kolkata, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 861-871 0932-0113 108:4<861 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer