caa a22 4500 445342757 CHVBK 20180317142818.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-010-2182-6 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2182-6 Expressed sequence tags from Eimeria brunetti— preliminary analysis and functional annotation [Elektronische Daten] [S. Aarthi, Gopal Raj, M. Raman, Damer Blake, Chandra Subramaniam, Fiona Tomley] As a first attempt to generate sequence information from the protein-coding genes of the genomically unknown parasite, Eimeria brunetti, a cDNA library was generated from purified sporozoites in the λTriplEx2™ vector. Analysis of 283 expressed sequence tags (ESTs) from the cDNA library constructed revealed 12 contigs (26 ESTs) and 257 singletons. BLASTx analysis revealed that 50 transcripts had significant matches to known proteins, whereas the remaining 233 had no significant matches, probably representing novel genes. Based on Gene Ontology classification, the transcripts were categorized as biological process (46 ESTs), molecular function (37 ESTs), and cellular component (19 ESTs). The transcripts analyzed show maximum homology to the apicomplexan parasite Toxoplasma gondii. Despite the small number of transcripts, this is the first transcriptome analysis of E. brunetti and provides preliminary data that will increase understanding of parasite biological function. Springer-Verlag, 2010 Aarthi S. Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, 600007, Chennai, India aut Raj Gopal Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, 600007, Chennai, India aut Raman M. Department of Veterinary Parasitology, MVC, 600007, Chennai, India aut Blake Damer Institute for Animal Health, Compton, UK aut Subramaniam Chandra Institute for Animal Health, Compton, UK aut Tomley Fiona Institute for Animal Health, Compton, UK aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 1059-1062 0932-0113 108:4<1059 2011 108 436 https://doi.org/10.1007/s00436-010-2182-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2182-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aarthi S. Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, 600007, Chennai, India aut NATIONALLICENCE 700 1- Raj Gopal Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, 600007, Chennai, India aut NATIONALLICENCE 700 1- Raman M. Department of Veterinary Parasitology, MVC, 600007, Chennai, India aut NATIONALLICENCE 700 1- Blake Damer Institute for Animal Health, Compton, UK aut NATIONALLICENCE 700 1- Subramaniam Chandra Institute for Animal Health, Compton, UK aut NATIONALLICENCE 700 1- Tomley Fiona Institute for Animal Health, Compton, UK aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 1059-1062 0932-0113 108:4<1059 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer