caa a22 4500 445342765 CHVBK 20180317142819.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-010-2145-y doi (NATIONALLICENCE)springer-10.1007/s00436-010-2145-y Imaging of Toxocara canis larvae labelled by CFSE in BALB/c mice [Elektronische Daten] [Petra Kolbeková, Libuše Kolářová, David Větvička, Martin Syrůček] Mice are used most often as a model for human toxocarosis caused by Toxocara canis larvae. Variety of symptoms developing during the infection reflects behaviour of the larvae, which are able to escape from the intestine and further invade and damage various host organs. In order to find an approach enabling observation on parasite behaviour in mouse in vivo, we used an epifluorescence method and a small animal imaging system (SAIS). Larvae of T. canis were labelled by carboxyfluorescein succinimidyl ester (CFSE) which incorporated on the parasite gastrointestinal tract. Following infection of BALB/c mice by CFSE-labelled larvae it has been observed that staining had no influence on viability and further migratory activity of the parasites through the host organs (the intestine, liver, lungs and brain) where they were detected by SAIS until day17 p.i. In addition, the dye did not affect larval antigenic activity as well as the development of related immune response. Imaging of parasites labelled by CFSE, therefore, may represent a promising way to study behaviour of T. canis larvae in a paratenic host. Springer-Verlag, 2010 Kolbeková Petra National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic aut Kolářová Libuše National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic aut Větvička David Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Science, Prague, Czech Republic aut Syrůček Martin Department of Pathology, Na Homolce Hospital, Prague, Czech Republic aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 1007-1014 0932-0113 108:4<1007 2011 108 436 https://doi.org/10.1007/s00436-010-2145-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2145-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kolbeková Petra National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic aut NATIONALLICENCE 700 1- Kolářová Libuše National Reference Laboratory for Tissue Helminthoses, General University Hospital in Prague, Prague, Czech Republic aut NATIONALLICENCE 700 1- Větvička David Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Science, Prague, Czech Republic aut NATIONALLICENCE 700 1- Syrůček Martin Department of Pathology, Na Homolce Hospital, Prague, Czech Republic aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 1007-1014 0932-0113 108:4<1007 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer