caa a22 4500 445342781 CHVBK 20180317142819.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-010-2148-8 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2148-8 Secondary structure analysis of ITS2 in the rDNA of three Indian paramphistomid species found in local livestock [Elektronische Daten] [Jollin Shylla, Sudeep Ghatani, Anupam Chatterjee, Veena Tandon] Of paramphistomid trematodes, three species viz., Homalogaster paloniae, Calicophoron calicophorum and Orthocoelium streptocoelium are commonly prevalent in bovine hosts in Northeast India. The aim of the present study was to genetically characterise these species using rDNA second internal transcribed spacer (ITS2) so as to supplement the morphological criteria substantiated by molecular findings. The annotated ITS2 region from H. paloniae, C. calicophorum and O. streptocoelium were found to be 289bp, 288bp and 288bp long, respectively. On comparison, the Indian isolates of the three species were observed to have a maximum identity of 99% with each of their respective counterparts from Japan. The secondary structure models were inferred using minimum free energy modelling algorithms. The paramphistomes displayed the typical four helix ITS2 secondary structure and differed from each other due to minor nucleotide differences. The consensus ITS2 secondary structure model revealed the presence of conservative motifs GACGAGGGUG and GCGGUAGAGUC in helix III. Monophyly is well supported for a clade consisting of the Japanese and Indian paramphistomes with significant bootstrap values. Springer-Verlag, 2010 Shylla Jollin Department of Zoology, North-Eastern Hill University, 793022, Shillong, Meghalaya, India aut Ghatani Sudeep Department of Zoology, North-Eastern Hill University, 793022, Shillong, Meghalaya, India aut Chatterjee Anupam Department of Biotechnology, North-Eastern Hill University, 793022, Shillong, Meghalaya, India aut Tandon Veena Department of Zoology, North-Eastern Hill University, 793022, Shillong, Meghalaya, India aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 1027-1032 0932-0113 108:4<1027 2011 108 436 https://doi.org/10.1007/s00436-010-2148-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2148-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shylla Jollin Department of Zoology, North-Eastern Hill University, 793022, Shillong, Meghalaya, India aut NATIONALLICENCE 700 1- Ghatani Sudeep Department of Zoology, North-Eastern Hill University, 793022, Shillong, Meghalaya, India aut NATIONALLICENCE 700 1- Chatterjee Anupam Department of Biotechnology, North-Eastern Hill University, 793022, Shillong, Meghalaya, India aut NATIONALLICENCE 700 1- Tandon Veena Department of Zoology, North-Eastern Hill University, 793022, Shillong, Meghalaya, India aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 1027-1032 0932-0113 108:4<1027 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer