caa a22 4500 44534279X CHVBK 20180317142819.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-010-2168-4 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2168-4 The effects of different plant extracts on nematodes [Elektronische Daten] [Sven Klimpel, Fathy Abdel-Ghaffar, Khaled Al-Rasheid, Gülendem Aksu, Katja Fischer, Bianca Strassen, Heinz Mehlhorn] The anthelminthic efficacy of some differently obtained extracts of several plants was tested in vivo in laboratory animals and in vitro. The extracts were obtained by ethanolic, methanolic, aqueous, or chloroform, respectively, acetonitrile polyethylenglycol (PEG) and/or propylencarbonate (PC) elution at room temperature or at 37°C. The plants used were bulbs of onions, garlic, chives, coconut, birch tree, ananas, cistrose, banana, chicory, date palm fruit, fig, pumpkin, and neem tree seeds. The worm systems tested both in vivo and in vitro were Trichuris muris and Angiostrongylus cantonensis but only in vivo Toxocara cati. The tests clearly showed that the different extraction methods eluted different components and different mass amounts, which had different efficacies against the above-cited worms. In vitro effects against A. cantonensis and T.muris were best with aqueous extracts, followed by chloroform extracts. The other plant extracts showed only low or no effects on A. cantonensis in vitro. In the case of T. muris, best results were obtained in vivo and in vitro with PEG/PC extracts of the onion followed by the aqueous extract of coconut. The complete elimination of worms in the in vivo experiments with T. muris was obtained when infected mice were treated with a 1:1 mixture of extracts of coconut and onion being produced by elutions with a mixture of 1:1 PEG and PC and fed daily for 8days. T. cati in a naturally infected cat was eliminated by daily oral application of 6ml coco's fluid for 5days. This study shows that a broad spectrum of plants has anti-nematodal activities, the intensity of which, however, depends on the mode of extraction. This implicates that, if results should be really comparable, the same extraction methods at the same temperatures have to be used. Furthermore, efficacy in in vitro systems does not guarantee as good—if at all—efficacy in vivo. Springer-Verlag, 2010 Klimpel Sven Biodiversity and Climate Change Center (BiK-F), Goethe-University Frankfurt, 60325, Frankfurt, Germany aut Abdel-Ghaffar Fathy Department of Zoology, Cairo University, Giza, Egypt aut Al-Rasheid Khaled Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia aut Aksu Gülendem Department of Zoology, Heinrich-Heine-University, 40225, Duesseldorf, Germany aut Fischer Katja Department of Zoology, Heinrich-Heine-University, 40225, Duesseldorf, Germany aut Strassen Bianca Department of Zoology, Heinrich-Heine-University, 40225, Duesseldorf, Germany aut Mehlhorn Heinz Department of Zoology, Heinrich-Heine-University, 40225, Duesseldorf, Germany aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 1047-1054 0932-0113 108:4<1047 2011 108 436 https://doi.org/10.1007/s00436-010-2168-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2168-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Klimpel Sven Biodiversity and Climate Change Center (BiK-F), Goethe-University Frankfurt, 60325, Frankfurt, Germany aut NATIONALLICENCE 700 1- Abdel-Ghaffar Fathy Department of Zoology, Cairo University, Giza, Egypt aut NATIONALLICENCE 700 1- Al-Rasheid Khaled Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia aut NATIONALLICENCE 700 1- Aksu Gülendem Department of Zoology, Heinrich-Heine-University, 40225, Duesseldorf, Germany aut NATIONALLICENCE 700 1- Fischer Katja Department of Zoology, Heinrich-Heine-University, 40225, Duesseldorf, Germany aut NATIONALLICENCE 700 1- Strassen Bianca Department of Zoology, Heinrich-Heine-University, 40225, Duesseldorf, Germany aut NATIONALLICENCE 700 1- Mehlhorn Heinz Department of Zoology, Heinrich-Heine-University, 40225, Duesseldorf, Germany aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 1047-1054 0932-0113 108:4<1047 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer