caa a22 4500 445342846 CHVBK 20180317142819.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-011-2286-7 doi (NATIONALLICENCE)springer-10.1007/s00436-011-2286-7 Immunoblot diagnosis of infection with Neospora caninum in cattle based on recombinant NcSAG4 Antigen [Elektronische Daten] [Jinqiang Hu, Ezio Ferroglio, Anna Trisciuoglio] The Neospora caninum (N. caninum) NcSAG4 gene was subcloned into a pET-28a (+) vector and successfully expressed in Escherichia coli as inclusion body, and confirmed by SDS-PAGE and Western blot using anti-His monoclonal antibody. The purified protein was then purified using Ni-NTA affinity chromatography column and recognized by positive serum from N. caninum-infected cattle. Immunoblot (IB) method based on purified recombinant NcSAG4 (rNcSAG4) antigen to detect antibodies against N. caninum in cattle was developed. Subsequently, both IB and ELISA kit were used to test sera (52) from naturally infected/uninfected cattle. Results showed that 50 and 48 out of 52 was positive for IB and ELISA kit, respectively, revealing that IB is more or at least as sensitive as ELISA when used for serodiagnosis of infected cattle Springer-Verlag, 2011 Hu Jinqiang Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università di Torino, No. 44 Via L. Da Vinci, 10095, Grugliasco, Torino, Italy aut Ferroglio Ezio Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università di Torino, No. 44 Via L. Da Vinci, 10095, Grugliasco, Torino, Italy aut Trisciuoglio Anna Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università di Torino, No. 44 Via L. Da Vinci, 10095, Grugliasco, Torino, Italy aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 1055-1058 0932-0113 108:4<1055 2011 108 436 https://doi.org/10.1007/s00436-011-2286-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-011-2286-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Jinqiang Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università di Torino, No. 44 Via L. Da Vinci, 10095, Grugliasco, Torino, Italy aut NATIONALLICENCE 700 1- Ferroglio Ezio Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università di Torino, No. 44 Via L. Da Vinci, 10095, Grugliasco, Torino, Italy aut NATIONALLICENCE 700 1- Trisciuoglio Anna Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Università di Torino, No. 44 Via L. Da Vinci, 10095, Grugliasco, Torino, Italy aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 1055-1058 0932-0113 108:4<1055 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer