caa a22 4500 445342889 CHVBK 20180317142819.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-010-2147-9 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2147-9 Molecular cloning and characterization of a novel ras-related protein (rap2) from Clonorchis sinensis [Elektronische Daten] [Wenjun Chen, Xiaoyun Wang, Chuanhuan Deng, Xiaoli Lv, Yongxiu Fan, Jingtao Men, Chi Liang, Xinbing Yu] Ras are key components of diverse signal transduction pathways and play important roles in growth and development. To know about growth regulation in Clonorchis sinensis, we have identified a full-length sequence encoding a ras-related protein (rap2) from our adult cDNA library. The open reading frame contains 561bp encoding 186 amino acids. The hypothetical amino acid sequence shared high identities with rap2 proteins from Schistosoma japonicum and Homo sapiens. Conserved domains of small guanosine triphosphate-binding proteins and characteristic amino acid residues of rap2 proteins were observed in this sequence. Reverse transcription polymerase chain reaction experiments revealed that rap2 transcribed in adult worm, metacercaria, and eggs of C. sinensis. Recombinant rap2 protein was expressed and purified from Escherichia coli. rap2 could be probed by C. sinensis-infected rat serum in western blotting experiment. By immunohistochemistry, rap2 was localized on the tegument of adult worm and metacercaria of C. sinensis. This fundamental study might contribute to further researches in signaling systems that are related to growth control and development of C. sinensis and other parasites. Springer-Verlag, 2010 Chen Wenjun Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut Wang Xiaoyun Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut Deng Chuanhuan Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut Lv Xiaoli Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut Fan Yongxiu Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut Men Jingtao Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut Liang Chi Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut Yu Xinbing Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 1021-1026 0932-0113 108:4<1021 2011 108 436 https://doi.org/10.1007/s00436-010-2147-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2147-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Wenjun Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Wang Xiaoyun Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Deng Chuanhuan Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Lv Xiaoli Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Fan Yongxiu Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Men Jingtao Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Liang Chi Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yu Xinbing Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd Road, 510080, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 1021-1026 0932-0113 108:4<1021 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer