caa a22 4500 445342935 CHVBK 20180317142819.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-010-2140-3 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2140-3 Molecular evidence of Opisthorchis viverrini in infected bithyniid snails in the Lao People's Democratic Republic by specific hybridization probe-based real-time fluorescence resonance energy transfer PCR method [Elektronische Daten] [Pusadee Sri-Aroon, Pewpan Intapan, Chantima Lohachit, Phunthira Phongsasakulchoti, Tongjit Thanchomnang, Viraphong Lulitanond, Alexandra Hiscox, Samlane Phompida, Pany Sananikhom, Wanchai Maleewong, Paul Brey] Naturally occurring bithyniid snails, Bithynia siamensis goniomphalos (Prosobranchia: Bithyniidae), and their intermediate hosts were sampled from Khammouane Province, the Lao People's Democratic Republic, and the prevalence of the carcinogenic human liver fluke, Opisthorchis viverrini, was examined. The presence of O. viverrini cercariae in snails was examined by cercarial shedding test and then confirmed by specific hybridization probe-based real-time fluorescence resonance energy transfer (FRET) PCR method. The real-time FRET PCR method is based on a fluorescence melting curve analysis of a hybrid between an amplicon produced from the pOV-A6 specific sequence (Genbank accession no. S80278), a 162-bp repeated sequence specific to O. viverrini, and specific fluorophore-labeled probes. Mean melting temperature of O. viverrini DNA from the cercariae and each of two positive snails by shedding test was 66.3 ± 0.1. The O. viverrini infection rate in snails was 2.47% (2/81) by cercarial shedding test but was 8.52% (4/47) by real-time FRET PCR method. The real-time FRET PCR method is rapid and effective in examining a large number of snail samples simultaneously. Validation using molecular evidence from this procedure provides another tool for surveying the prevalence of O. viverrini-infected snails in Southeast Asian countries. Springer-Verlag, 2010 Sri-Aroon Pusadee Applied Malacology Center, Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, 10400, Bangkok, Thailand aut Intapan Pewpan Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, 40002, Khon Kaen, Thailand aut Lohachit Chantima Applied Malacology Center, Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, 10400, Bangkok, Thailand aut Phongsasakulchoti Phunthira Applied Malacology Center, Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, 10400, Bangkok, Thailand aut Thanchomnang Tongjit Faculty of Medicine, Mahasarakham University, 44150, Mahasarakham, Thailand aut Lulitanond Viraphong Department of Microbiology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, 40002, Khon Kaen, Thailand aut Hiscox Alexandra Institut Pasteur du Laos, Vientiane, Lao People's Democratic Republic aut Phompida Samlane National Center for Malariology, Parasitology and Entomology, Ministry of Health, Vientiane, Lao People's Democratic Republic aut Sananikhom Pany Health Program Management Unit, Nam Theun 2 Power Company, Khammouane, Lao People's Democratic Republic aut Maleewong Wanchai Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, 40002, Khon Kaen, Thailand aut Brey Paul Institut Pasteur du Laos, Vientiane, Lao People's Democratic Republic aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 973-978 0932-0113 108:4<973 2011 108 436 https://doi.org/10.1007/s00436-010-2140-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2140-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sri-Aroon Pusadee Applied Malacology Center, Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Intapan Pewpan Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Lohachit Chantima Applied Malacology Center, Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Phongsasakulchoti Phunthira Applied Malacology Center, Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, 10400, Bangkok, Thailand aut NATIONALLICENCE 700 1- Thanchomnang Tongjit Faculty of Medicine, Mahasarakham University, 44150, Mahasarakham, Thailand aut NATIONALLICENCE 700 1- Lulitanond Viraphong Department of Microbiology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Hiscox Alexandra Institut Pasteur du Laos, Vientiane, Lao People's Democratic Republic aut NATIONALLICENCE 700 1- Phompida Samlane National Center for Malariology, Parasitology and Entomology, Ministry of Health, Vientiane, Lao People's Democratic Republic aut NATIONALLICENCE 700 1- Sananikhom Pany Health Program Management Unit, Nam Theun 2 Power Company, Khammouane, Lao People's Democratic Republic aut NATIONALLICENCE 700 1- Maleewong Wanchai Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, 40002, Khon Kaen, Thailand aut NATIONALLICENCE 700 1- Brey Paul Institut Pasteur du Laos, Vientiane, Lao People's Democratic Republic aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 973-978 0932-0113 108:4<973 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer