caa a22 4500 445342994 CHVBK 20180317142819.0 cr unu---uuuuu 170323e20110401xx s 000 0 eng 10.1007/s00436-010-2129-y doi (NATIONALLICENCE)springer-10.1007/s00436-010-2129-y Effects of curcumin (diferuloylmethane) on Eimeria tenella sporozoites in vitro [Elektronische Daten] [Reda Khalafalla, Uwe Müller, Md Shahiduzzaman, Viktor Dyachenko, Abdelrazik Desouky, Gottfried Alber, Arwid Daugschies] The negative effects of coccidiosis on poultry health and productivity and increasing problems related to drug resistance have stimulated the search for novel and alternative methods of control. The present study evaluates the anticoccidial activity of curcumin (diferuloylmethane), a natural polyphenolic compound abundant in the rhizome of the perennial herb turmeric (Curcuma longa) which is a spice and food colourant commonly used in curries and also used as medicinal herb. Its effects were evaluated on Eimeria tenella sporozoites, including morphological alterations, sporozoite viability and infectivity to Madin-Darby bovine kidney (MDBK) cells. Morphological alterations of the sporozoites were recorded as deformation due to swelling and cell membrane corrugations. Curcumin at concentrations of 25, 50, 100, 200 and 400μM showed considerable effects on sporozoite morphology and viability in a dose-dependent manner after incubation over 3, 6, 18 and 24h while lower curcumin concentrations (6.25 and 12.5μM) were not effective. In comparison to the untreated control, sporozoite infectivity was reduced at curcumin concentrations of 100 and 200μM by 41.6% and 72.8%, respectively. Negative effects of curcumin on MDBK cells were not seen at these concentrations; however, curcumin at concentrations of 1,800, 600 and 400μM was toxic to MDBK cells and affected cell proliferation. In conclusion, curcumin exhibited a marked inhibitory effect in vitro on E. tenella sporozoites inducing morphological changes and reducing sporozoite viability and infectivity. Springer-Verlag, 2010 Khalafalla Reda Institute of Parasitology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut Müller Uwe Institute of Immunology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut Shahiduzzaman Md Institute of Parasitology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut Dyachenko Viktor Institute of Parasitology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut Desouky Abdelrazik Parasitology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt aut Alber Gottfried Institute of Immunology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut Daugschies Arwid Institute of Parasitology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut Parasitology Research Springer-Verlag 108/4(2011-04-01), 879-886 0932-0113 108:4<879 2011 108 436 https://doi.org/10.1007/s00436-010-2129-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2129-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khalafalla Reda Institute of Parasitology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut NATIONALLICENCE 700 1- Müller Uwe Institute of Immunology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut NATIONALLICENCE 700 1- Shahiduzzaman Md Institute of Parasitology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut NATIONALLICENCE 700 1- Dyachenko Viktor Institute of Parasitology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut NATIONALLICENCE 700 1- Desouky Abdelrazik Parasitology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt aut NATIONALLICENCE 700 1- Alber Gottfried Institute of Immunology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut NATIONALLICENCE 700 1- Daugschies Arwid Institute of Parasitology, Faculty of Veterinary Medicine, University Leipzig, Leipzig, Germany aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/4(2011-04-01), 879-886 0932-0113 108:4<879 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer