caa a22 4500 445343036 CHVBK 20180317142819.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00436-010-2157-7 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2157-7 Small subunit ribosomal RNA and mitochondrial cytochrome c oxidase subunit 1 gene sequences of 21 strains of the parasitic scuticociliate Miamiensis avidus (Ciliophora, Scuticociliatia) [Elektronische Daten] [Sung-Ju Jung, Eun-Young Im, Michaela Strüder-Kypke, Shin-Ichi Kitamura, Patrick Woo] The scuticociliate Miamiensis avidus is a histophagous parasite that causes high mortality in cultured marine fishes. Small subunit ribosomal RNA (SSU rRNA) and mitochondrial cytochrome c oxidase subunit 1 (cox1) genes were analyzed for 21 strains of M. avidus isolated from diseased olive flounder (Paralichthys olivaceus), ridged-eye flounder (Pleuronichthys cornutus), and spotted knifejaw (Oplegnathus fasciatus) in Korea and Japan (collected in 2003-2007). Analysis of SSU rRNA gene sequences (1,759bp) indicates they are very conserved with less than 0.17% (3 nucleotides) differences suggesting that SSU rRNA are useful to identify M. avidus; however, the cox1 gene (900bp) has higher variations with intraspecific divergences up to 5.67% (51 nucleotides). A distance tree of cox1 gene sequences based on a neighbor-joining analysis can separate 21 strains into five cox1 types (two heterogeneous clusters and three individual branches). The cox1-type matches with serotype of strains but do not reflect geographical origins, host species, or pathogenicity. Springer-Verlag, 2010 Jung Sung-Ju Department of Aqualife Medicine, Chonnam National University, 550-749, Chonnam, South Korea aut Im Eun-Young Department of Aqualife Medicine, Chonnam National University, 550-749, Chonnam, South Korea aut Strüder-Kypke Michaela Department of Integrative Biology, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Kitamura Shin-Ichi Center for Marine Environmental Studies (CMES), Ehime University, 790-8577, Matsuyama, Japan aut Woo Patrick Department of Integrative Biology, University of Guelph, N1G 2W1, Guelph, ON, Canada aut Parasitology Research Springer-Verlag 108/5(2011-05-01), 1153-1161 0932-0113 108:5<1153 2011 108 436 https://doi.org/10.1007/s00436-010-2157-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2157-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jung Sung-Ju Department of Aqualife Medicine, Chonnam National University, 550-749, Chonnam, South Korea aut NATIONALLICENCE 700 1- Im Eun-Young Department of Aqualife Medicine, Chonnam National University, 550-749, Chonnam, South Korea aut NATIONALLICENCE 700 1- Strüder-Kypke Michaela Department of Integrative Biology, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 700 1- Kitamura Shin-Ichi Center for Marine Environmental Studies (CMES), Ehime University, 790-8577, Matsuyama, Japan aut NATIONALLICENCE 700 1- Woo Patrick Department of Integrative Biology, University of Guelph, N1G 2W1, Guelph, ON, Canada aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/5(2011-05-01), 1153-1161 0932-0113 108:5<1153 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer