caa a22 4500 445343079 CHVBK 20180317142820.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s00436-010-2205-3 doi (NATIONALLICENCE)springer-10.1007/s00436-010-2205-3 Changes in the activity and kinetics of mouse intestinal glutathione transferase during experimental trichinellosis [Elektronische Daten] [Agnieszka Wojtkowiak-Giera, Elżbieta Wandurska-Nowak, Michał Michalak, Monika Derda] The glutathione S-transferases (GSTs) are a group of multifunctional enzymes, which play a critical role in cellular detoxification. Our investigations deal with the contribution of GST in the biochemical defense against Trichinella spiralis infection. The aim of this study was to examine the effect of T. spiralis infection on the total activity and kinetic properties of cytosolic GST in the intestine during the intestinal phase of experimental trichinellosis in mice. Our results showed a statistically significant increase (relative to the uninfected control) in the total GST activity both in the small and large intestines of the infected mice. Moreover, we observed changes in the kinetics of substrate saturation of GST. Trichinellosis in the small intestine caused a 12-fold decrease in the low K m value and a sixfold increase in the high K m value. In the large intestine, infection with T. spiralis caused only a fivefold increase in the low K m value, whereas the high K m value remained unchanged. We suggest that GST from the mouse small intestine could be involved in the detoxification of parasite excretory-secretory products released to the host intestine during trichinellosis and that these products influence the quaternary structure of this enzyme. Springer-Verlag, 2010 Wojtkowiak-Giera Agnieszka Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701, Poznan, Poland aut Wandurska-Nowak Elżbieta Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701, Poznan, Poland aut Michalak Michał Department of Computer Science and Statistics, Poznan University of Medical Sciences, Dabrowskiego 79, 60-529, Poznan, Poland aut Derda Monika Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701, Poznan, Poland aut Parasitology Research Springer-Verlag 108/5(2011-05-01), 1309-1313 0932-0113 108:5<1309 2011 108 436 https://doi.org/10.1007/s00436-010-2205-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s00436-010-2205-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wojtkowiak-Giera Agnieszka Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701, Poznan, Poland aut NATIONALLICENCE 700 1- Wandurska-Nowak Elżbieta Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701, Poznan, Poland aut NATIONALLICENCE 700 1- Michalak Michał Department of Computer Science and Statistics, Poznan University of Medical Sciences, Dabrowskiego 79, 60-529, Poznan, Poland aut NATIONALLICENCE 700 1- Derda Monika Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, Fredry 10, 61-701, Poznan, Poland aut NATIONALLICENCE 773 0- Parasitology Research Springer-Verlag 108/5(2011-05-01), 1309-1313 0932-0113 108:5<1309 2011 108 436 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer